Geyer H, Geyer R
Institute of Biochemistry, University of Giessen, Germany.
Acta Anat (Basel). 1998;161(1-4):18-35. doi: 10.1159/000046449.
Glycoconjugates such as glycoproteins and glycosphingolipids exhibit multiple functions in biological systems. A correlation of functional features with defined structural parameters, however, presupposes detailed information on the glycoconjugates' carbohydrate moieties including the type of linkage of the oligosaccharide chain(s), monosaccharide composition as well as sequence, linkage positions and anomeric configurations of individual sugar monomers. Chemical and biochemical analyses are often impeded by the limited amounts of sample available and the vast structural heterogeneity of glycoconjugate glycans, thus requiring highly sensitive and efficient methods for detection, separation and structural elucidation of these carbohydrates. The aim of the article is to present suitable strategies for structural characterization of glycoconjugate glycans and to briefly review some of the techniques commonly used in this field.
糖蛋白和糖鞘脂等糖缀合物在生物系统中具有多种功能。然而，功能特征与特定结构参数之间的关联，预先假定了有关糖缀合物碳水化合物部分的详细信息，包括寡糖链的连接类型、单糖组成以及单个糖单体的序列、连接位置和异头构型。化学和生化分析常常受到可用样品量有限以及糖缀合物聚糖巨大的结构异质性的阻碍，因此需要用于这些碳水化合物检测、分离和结构解析的高灵敏度和高效方法。本文的目的是介绍用于糖缀合物聚糖结构表征的合适策略，并简要回顾该领域常用的一些技术。
