[微核试验在使用人癌细胞系的放射敏感性测试中的实用性]

[Usefulness of micronucleus assay in radiosensitivity tests using human cancer cell lines].

作者信息

Kimura H

机构信息

Department of Urology, Faculty of Medicine, Toyama Medical and Pharmaceutical University.

出版信息

Nihon Hinyokika Gakkai Zasshi. 1998 Aug;89(8):712-20. doi: 10.5980/jpnjurol1989.89.712.

DOI:10.5980/jpnjurol1989.89.712

PMID:9780656

Abstract

BACKGROUND

Recently, micronucleus assay is expected to be one of the radiosensitivity tests. The usefulness of micronucleus assay was compared with MTT assay and clonogenic assay using 5 human derived urological cancer cell lines, NBT-2, T24, PC3, OS-RC-2, and RERF-LC-AI in vitro. The correlation between the results in vitro assay and the radiation effects of nude mouse in vivo was investigated.

METHODS

In vitro, the micronucleus frequency of 2 Gy radiation was scored in micronucleus assay. The survival fraction of 2 Gy radiation was obtained in MTT assay and clonogenic assay. The correlation between 3 assays was investigated. In vivo, cancer cells was inoculated to nude mouse and the tumor volume was measured at 3-7 days interval in control group and 10 Gy irradiated group. The tumor volume ratio in irradiated group to control group was calculated as a radiation effect in each cell lines, the correlation between this ratio in vivo and each value of 2 Gy radiation in vitro was studied.

RESULTS

The correlation between micronucleus frequency and survival fraction in clonogenic assay was statistically significant (r = 0.941, p = 0.0169). But the correlation of the survival fraction between MTT assay and clonogenic assay is not statistically significant. The correlation between micronucleus frequency and the tumor volume ratio in vivo was statistically significant (r = 0.990, p = 0.0011). The correlation between survival fraction in clonogenic assay and the tumor volume ratio in vivo was also statistically significant (r = 0.914, p = 0.0298). However, the correlation between survival fraction in MTT assay and the tumor volume ratio in vivo was not statistically significant (r = 0.782, p = 0.118).

CONCLUSION

In this 5 cell lines, micronucleus assay was most correlated to nude mouse radiation effect. This result suggested the possibility of micronucleus assay to be a better predictive method than clonogenic assay for radiosensitivity test.

摘要

背景

最近，微核试验有望成为放射敏感性测试之一。使用5种人源性泌尿系统癌细胞系NBT-2、T24、PC3、OS-RC-2和RERF-LC-AI在体外比较了微核试验与MTT试验和克隆形成试验的有效性。研究了体外试验结果与裸鼠体内辐射效应之间的相关性。

方法

在体外，微核试验中对2 Gy辐射的微核频率进行评分。MTT试验和克隆形成试验中获得2 Gy辐射的存活分数。研究了3种试验之间的相关性。在体内，将癌细胞接种到裸鼠体内，对照组和10 Gy照射组每隔3-7天测量肿瘤体积。计算照射组与对照组的肿瘤体积比作为每种细胞系的辐射效应，研究体内该比值与体外2 Gy辐射的各值之间的相关性。

结果

克隆形成试验中微核频率与存活分数之间的相关性具有统计学意义（r = 0.941，p = 0.0169）。但MTT试验与克隆形成试验的存活分数相关性无统计学意义。微核频率与体内肿瘤体积比之间的相关性具有统计学意义（r = 0.990，p = 0.0011）。克隆形成试验中的存活分数与体内肿瘤体积比之间的相关性也具有统计学意义（r = 0.914，p = 0.0298）。然而，MTT试验中的存活分数与体内肿瘤体积比之间的相关性无统计学意义（r = 0.782，p = 0.118）。