Enomoto T, Takao S, Mure H, Baba M, Aikou T
First Dept. of Surgery, Kagoshima University School of Medicine, Japan.
J Exp Clin Cancer Res. 1997 Sep;16(3):273-80.
After devising a 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-Hybrid assay that incorporates the advantages of both the clonogenic and standard MTT assays, we evaluated the validity of this system using a malignant fibrous histiocytoma (MFH) cell and four xenografted tumors. In the MFH cells the surviving fractions were correlated with radiation dose at three assays. In xenografted tumor cells, the surviving fractions determined by the MTT assay did not show a similar dose response, possibly because of contamination by normal cells. Changes in the surviving fraction of the xenografted cells were correlated to the radiation dose as determined by the clonogenic assays, but this assay took longer performance times and was not useful for evaluating tumors with a low planting efficiency. With the MTT-hybrid assay, changes in fractions of the four xenografted tumor cells were correlated to radiation dose. The MTT-hybrid assay required a smaller optimal number seeding cells and shorter assay time than those of the clonogenic assay. Therefore, the MTT-Hybrid assay can be used successfully to assess the radiosensitivity of both established tumor cell lines and fresh tumor samples.
在设计出一种结合了克隆形成试验和标准MTT试验优点的3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐(MTT)混合试验后，我们使用恶性纤维组织细胞瘤(MFH)细胞和四种异种移植肿瘤评估了该系统的有效性。在MFH细胞中，三种试验的存活分数与辐射剂量相关。在异种移植肿瘤细胞中，MTT试验测定的存活分数未显示出类似的剂量反应，这可能是由于正常细胞的污染。异种移植细胞存活分数的变化与克隆形成试验确定的辐射剂量相关，但该试验耗时较长，对于评估种植效率低的肿瘤并无用处。采用MTT混合试验时，四种异种移植肿瘤细胞的分数变化与辐射剂量相关。与克隆形成试验相比，MTT混合试验所需的最佳接种细胞数量更少，试验时间更短。因此，MTT混合试验可成功用于评估已建立的肿瘤细胞系和新鲜肿瘤样本的放射敏感性。
