Slavotinek A, McMillan T J, Steel C M
MRC Human Genetics Unit, Western General Hospital, Edinburgh, U.K.
Eur J Cancer. 1994;30A(9):1376-82. doi: 10.1016/0959-8049(94)90189-9.
There is increasing interest in the development of rapid assays of radiosensitivity which can be used on clinical specimens. Unfortunately, the measurement of radiation survival using clonogenic assays, which are the established standard, can be difficult and time consuming. We have used the MTT assay to measure the radiation survival of four lymphoblastoid cell lines with low plating efficiencies. We measured surviving fractions both when the irradiated cells had regained exponential growth and when the non-irradiated cells had undergone four or more doublings. The results were compared to surviving fractions measured by clonogenic assay. We found both methods could be used successfully to rank the cell lines in order of radiosensitivity. However, cells exposed to the higher radiation doses in the MTT assay did not always regain exponential growth, limiting the dose range for which the assay was useful. We also found the best correlation between the two assays was sometimes obtained by using the MTT surviving fractions from different days for different radiation dose levels. Thus, although the MTT assay can be used to measure radiation survival in relation to other cell lines, its use can be complicated by restrictions on radiation dose ranges and difficulties with data interpretation.
人们对能够用于临床样本的放射敏感性快速检测方法的开发越来越感兴趣。不幸的是，使用作为既定标准的克隆形成试验来测量辐射存活率可能既困难又耗时。我们使用MTT试验来测量四种接种效率低的淋巴母细胞系的辐射存活率。我们在辐照细胞恢复指数生长时以及未辐照细胞经历四次或更多次倍增时测量存活分数。将结果与通过克隆形成试验测量的存活分数进行比较。我们发现两种方法都可以成功地用于按放射敏感性对细胞系进行排序。然而，在MTT试验中暴露于较高辐射剂量的细胞并不总是能恢复指数生长，这限制了该试验有用的剂量范围。我们还发现，有时通过使用不同辐射剂量水平不同天数的MTT存活分数，可以在两种试验之间获得最佳相关性。因此，尽管MTT试验可用于测量相对于其他细胞系的辐射存活率，但其使用可能会因辐射剂量范围的限制和数据解释的困难而变得复杂。
