Barlow A L, Hultén M A
LSF Research Unit, Heartlands Hospital, Birmingham, UK.
Eur J Hum Genet. 1998 Jul-Aug;6(4):350-8. doi: 10.1038/sj.ejhg.5200200.
The distribution of anti-MLH1 (MutL homologue 1) antibody labelling was studied in human prophase meiocytes. A labelling pattern consisting of distinct foci, always associated with the synaptonemal complex (SC) and never in closely juxtaposed pairs, was observed. Comparison of the number and general positions of autosomal foci with previous studies of the number and positions of autosomal chiasmata indicates that the anti-MLH1 antibody marks sites of crossing over in human pachytene spermatocytes. A mean number of 50.9 autosomal foci was observed from 46 human pachytene spermatocytes corresponding to a genetic length of 2545 cm. Division of these spermatocytes into sub-stages revealed that the number of foci remains stable throughout pachytene. A focus was found on the XY bivalent in 56.5% of the nuclei. The presence or absence of foci from the XY bivalent could not be correlated to pachytene sub-stage.
在人类减数分裂前期细胞中研究了抗MLH1（MutL同源物1）抗体标记的分布。观察到一种由不同的焦点组成的标记模式，这些焦点总是与联会复合体（SC）相关，且从不紧密相邻成对出现。将常染色体焦点的数量和大致位置与先前关于常染色体交叉点数量和位置的研究进行比较，表明抗MLH1抗体标记了人类粗线期精母细胞中的交叉位点。从46个人类粗线期精母细胞中观察到平均50.9个常染色体焦点，对应于2545厘米的遗传长度。将这些精母细胞分为不同亚阶段，结果显示在整个粗线期焦点数量保持稳定。在56.5%的细胞核中，XY二价体上发现了一个焦点。XY二价体上焦点的有无与粗线期亚阶段无关。
