Work T M, Raskin R E, Balazs G H, Whittaker S D
US Geological Survey, Biological Resource Division, National Wildlife Health Center, Honolulu, HI 96850, USA.
Am J Vet Res. 1998 Oct;59(10):1252-7.
To identify and characterize blood cells from free-ranging Hawaiian green turtles, Chelonia mydas.
26 green turtles from Puako on the island of Hawaii and Kaneohe Bay on the island of Oahu.
Blood was examined, using light and electron microscopy and cytochemical stains that included benzidine peroxidase, chloroacetate esterase, alpha naphthyl butyrate esterase, acid phosphatase, Sudan black B, periodic acid-Schiff, and toluidine blue.
6 types of WBC were identified: lymphocytes, monocytes, thrombocytes, heterophils, basophils, and eosinophils (small and large). Morphologic characteristics of mononuclear cells and most granulocytes were similar to those of cells from other reptiles except that green turtles have both large and small eosinophils.
Our classification of green turtle blood cells clarifies improper nomenclature reported previously and provides a reference for future hematologic studies in this species.
鉴定和描述野生夏威夷绿海龟（蠵龟）的血细胞。
来自夏威夷岛普阿科和瓦胡岛卡内奥赫湾的26只绿海龟。
使用光学显微镜、电子显微镜和细胞化学染色法检查血液，细胞化学染色包括联苯胺过氧化物酶、氯乙酸酯酶、α-萘丁酸酯酶、酸性磷酸酶、苏丹黑B、过碘酸-希夫反应和甲苯胺蓝。
鉴定出6种白细胞类型：淋巴细胞、单核细胞、血小板、异嗜性粒细胞、嗜碱性粒细胞和嗜酸性粒细胞（大型和小型）。单核细胞和大多数粒细胞的形态特征与其他爬行动物的细胞相似，只是绿海龟既有大型嗜酸性粒细胞也有小型嗜酸性粒细胞。
我们对绿海龟血细胞的分类澄清了先前报道的不当命名，并为该物种未来的血液学研究提供了参考。
