用于在丝状噬菌体表面展示肽的新型载体。

New vectors for peptide display on the surface of filamentous bacteriophage.

作者信息

Malik P, Perham R N

机构信息

Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK.

出版信息

Gene. 1996 May 24;171(1):49-51. doi: 10.1016/0378-1119(96)00070-4.

DOI:10.1016/0378-1119(96)00070-4

PMID:8675029

Abstract

We have modified the genome of the filamentous bacteriophage fd and also constructed a number of new vectors for the purpose of displaying peptides on the surface of the virion. These vectors facilitate the directional cloning of DNA encoding a peptide of interest at or near the N terminus of the major coat protein, the product of the bacteriophage gene VIII, and the construction of hybrid capsids in which the modified coat protein is interspersed with wild-type coat protein subunits.

摘要

我们已经对丝状噬菌体fd的基因组进行了改造，并构建了一些新载体，目的是在病毒粒子表面展示肽段。这些载体有助于将编码感兴趣肽段的DNA定向克隆到噬菌体基因VIII产物主要衣壳蛋白的N端或其附近，并构建杂合衣壳，其中修饰后的衣壳蛋白与野生型衣壳蛋白亚基相间排列。