Longworth J F, Carey G P
J Gen Virol. 1976 Oct;33(1):31-40. doi: 10.1099/0022-1317-33-1-31.
Some properties of a small RNA virus isolated from larvae of Heteronychus arator [Coleoptera: Scarabaeidae] are described. The virus develops in the cytoplasm of gut and fat body cells and is 30 nm in diamter. The sedimentation coefficient of virus was 137S and the buoyant density was 1-33 g/ml. The single stranded RNA was isolated as two species which sedimented at 22S and 15S, corresponding to mol. wt. of 1-0 and 0-5 X 10(6) respectively. The base ratios of the two RNA species were 22 S: A=29-0, C=24-1, G¿-8, U=24-1; 15S:A=26-4, C=23-8, G=24-7, U=25-2. There was one major polypeptide, mol. wt. 40 000 and two minor polypeptides in the virus. The virus was infective for Galleria mellonella and some other insects, but was not infective for mice. No evidence was obtained to suggest that the two RNA species were present in different particles.
描述了从甘蔗象甲幼虫[鞘翅目：金龟科]中分离出的一种小RNA病毒的一些特性。该病毒在肠道和脂肪体细胞的细胞质中发育，直径为30纳米。病毒的沉降系数为137S，浮力密度为1.33克/毫升。单链RNA被分离为两种，沉降系数分别为22S和15S，分子量分别为1.0×10⁶和0.5×10⁶。两种RNA的碱基比例分别为：22S：A = 29.0，C = 24.1，G = 8，U = 24.1；15S：A = 26.4，C = 23.8，G = 24.7，U = 25.2。病毒中有一种主要多肽，分子量为40000，还有两种次要多肽。该病毒对大蜡螟和其他一些昆虫具有感染性，但对小鼠没有感染性。没有证据表明这两种RNA存在于不同的颗粒中。
