细胞分裂素4PU30对甘茶愈伤组织培养中器官分化和二氢异香豆素产生的影响。

Effect of cytokinin 4PU30 on organ differentiation and dihydroisocoumarin production in amacha callus cultures.

作者信息

Yamamoto H, Yamamoto M, Yamamoto K, Chatani N, Yoshikawa M

机构信息

Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.

出版信息

Biol Pharm Bull. 1998 Sep;21(9):1003-4. doi: 10.1248/bpb.21.1003.

DOI:10.1248/bpb.21.1003

PMID:9781858

Abstract

Effect of a suitable combination of 1-naphthaleneacetic acid (NAA) and cytokinin N-(2-chloro-4-pyridyl)-N'-phenylurea (4PU30) concentrations, regarding organ differentiation and dihydroisocoumarin production, was examined in Hydrangea macrophylla var. thunbergii callus cultures. The callus cultured in a Linsmaier-Skoog medium containing 10(-5) M NAA and 10(-5) M 4PU30 under a light condition gave the highest level for callus growth, shoot formation, and phyllodulcin 8-O-glucoside content.

摘要

在绣球花变种（Hydrangea macrophylla var. thunbergii）愈伤组织培养中，研究了1-萘乙酸（NAA）和细胞分裂素N-(2-氯-4-吡啶基)-N'-苯基脲（4PU30）浓度的合适组合对器官分化和二氢异香豆素产生的影响。在光照条件下，在含有10(-5) M NAA和10(-5) M 4PU30的Linsmaier-Skoog培养基中培养的愈伤组织，其愈伤组织生长、芽形成和根皮苷8-O-葡萄糖苷含量达到最高水平。