乙酰胆碱释放。基本量子机制的重建。

Acetylcholine release. Reconstitution of the elementary quantal mechanism.

作者信息

Israël M, Dunant Y

机构信息

Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, Gif-sur-Yvette, France.

出版信息

J Physiol Paris. 1998 Apr;92(2):123-8. doi: 10.1016/S0928-4257(98)80149-6.

DOI:10.1016/S0928-4257(98)80149-6

PMID:9782455

Abstract

Choline acetyltransferase and vesicular acetylcholine transporter genes are the products of two adjacent genes defining a cholinergic locus. The release mechanism is expressed independently of this locus in some cell lines. A cholinergic neuron will therefore have to coordinate the expression of release with that of the cholinergic locus. Transfection of a plasmid encoding Torpedo mediatophore in cells that are unable to release this transmitter endows them with a Ca2(+)-dependent and quantal release mechanism. The synchronization of mediatophore activation results from a control of calcium microdomains by the synaptic vesicles. It is therefore dependent on the proteins that dock vesicles close to calcium channels.

摘要

胆碱乙酰转移酶和囊泡乙酰胆碱转运体基因是定义胆碱能位点的两个相邻基因的产物。在一些细胞系中，释放机制独立于该位点表达。因此，胆碱能神经元必须协调释放与胆碱能位点的表达。在无法释放这种递质的细胞中，转染编码电鳐介质载体的质粒可赋予它们一种Ca2(+)依赖性的量子释放机制。介质载体激活的同步性源于突触囊泡对钙微区的控制。因此，它依赖于将囊泡停靠在钙通道附近的蛋白质。