Oberste M S, Maher K, Pallansch M A
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Virus Res. 1998 Aug;56(2):217-23. doi: 10.1016/s0168-1702(98)00080-x.
To define the relationship between echovirus 23 (E23) and other human enteroviruses, we have determined the complete nucleotide sequence of a strain of E23 isolated from a child with high fever in Connecticut in 1986 and compared the nucleotide and deduced amino acid sequences with those of other enteroviruses representing each of the major enterovirus phylogenetic groups, poliovirus type 1, coxsackievirus A16, coxsackievirus B3, echovirus 22 (E22), and enterovirus 70. The genome of E23 (strain CT86-6760) was 7352 nucleotides in length, exclusive of the poly(A) tail, and the genome organization was typical of the picornaviruses. The nucleotide sequence and deduced amino acid sequences were most related to those of E22, a virus with which E23 shares many biological properties, and was quite divergent from the sequences of other enteroviruses (< 20% average amino sequence identity). These data lend further support to the suggestion that E22 and E23 are distinct from members of the Enterovirus genus and that they should be reclassified in a separate genus within the Picornaviridae.
为了确定埃可病毒23型（E23）与其他人类肠道病毒之间的关系，我们测定了1986年从康涅狄格州一名高热儿童中分离出的一株E23的完整核苷酸序列，并将其核苷酸序列和推导的氨基酸序列与代表每个主要肠道病毒系统发育组的其他肠道病毒（脊髓灰质炎病毒1型、柯萨奇病毒A16、柯萨奇病毒B3、埃可病毒22型（E22）和肠道病毒70型）的序列进行了比较。E23（CT86 - 6760株）的基因组长度为7352个核苷酸，不包括聚腺苷酸尾，其基因组结构是典型的小核糖核酸病毒。核苷酸序列和推导的氨基酸序列与E22的序列关系最为密切，E23与E22具有许多生物学特性，并且与其他肠道病毒的序列差异很大（平均氨基酸序列同一性<20%）。这些数据进一步支持了以下观点：E22和E23与肠道病毒属的成员不同，它们应在小核糖核酸病毒科内的一个单独属中重新分类。
