Youssef P P, Smeets T J, Bresnihan B, Cunnane G, Fitzgerald O, Breedveld F, Tak P P
University College Dublin Department of Rheumatology, St Vincent's Hospital, Ireland.
Br J Rheumatol. 1998 Sep;37(9):1003-7. doi: 10.1093/rheumatology/37.9.1003.
Microscopic measurement of inflammation in synovial tissue may be important in studies of clinical status, prognosis and response to treatment. The aim of this study was to compare quantitative microscopic analysis of inflammation with a semiquantitative grading system in rheumatoid arthritis (RA) synovial membrane. Knee synovial membrane samples from 16 patients with RA, including paired samples taken before and after treatment in nine patients, were immunostained with anti-CD68 and anti-CD3 monoclonal antibodies using standard techniques. The intensity of macrophage and T-lymphocyte infiltration was measured both by quantitative and semiquantitative techniques, and the results were compared. In a cross-sectional comparison, both methods correlated significantly for lining layer macrophage infiltration, as well as sublining layer macrophage and T-cell infiltration. However, in some patients demonstrating a clinical response to treatment, semiquantitative analysis lacked sensitivity to biologically relevant changes in mononuclear cell infiltration. These observations have important implications for future studies of therapeutic modalities.
滑膜组织炎症的微观测量在临床状态、预后及治疗反应的研究中可能具有重要意义。本研究的目的是比较类风湿关节炎（RA）滑膜中炎症的定量微观分析与半定量分级系统。采用标准技术，用抗CD68和抗CD3单克隆抗体对16例RA患者的膝关节滑膜样本进行免疫染色，其中9例患者的样本为治疗前后的配对样本。通过定量和半定量技术测量巨噬细胞和T淋巴细胞浸润强度，并比较结果。在横断面比较中，两种方法对于衬里层巨噬细胞浸润、以及衬里下层巨噬细胞和T细胞浸润均具有显著相关性。然而，在一些显示出对治疗有临床反应的患者中，半定量分析对单核细胞浸润的生物学相关变化缺乏敏感性。这些观察结果对未来治疗方式的研究具有重要意义。
