Chu S, Herskowitz I
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448, USA.
Mol Cell. 1998 Apr;1(5):685-96. doi: 10.1016/s1097-2765(00)80068-4.
Gametogenesis requires the successful coordination of two key processes, meiotic nuclear division and gamete morphogenesis. A central regulatory step in progression through gametogenesis occurs at the pachytene stage of meiotic prophase. We find that Ndt80 functions at pachytene of yeast gametogenesis (sporulation) to activate transcription of a set of genes required for both meiotic division (e.g., B-type cyclins) and gamete formation (e.g., SPS1). Ectopic synthesis of Ndt80 in vegetative cells induces transcription of these genes, and recombinant Ndt80 protein binds to a conserved sequence in their upstream region. Transcription of NDT80 itself is dependent on Ime1, which activates expression of early sporulation genes. Transcription of the Ndt80-regulated gene CLB1 is mediated by the checkpoint gene RAD17. Thus Ndt80 is a pivotal component of a transcriptional cascade programming yeast gametogenesis and may also be a target of meiotic checkpoint control.
配子发生需要成功协调两个关键过程，即减数分裂核分裂和配子形态发生。配子发生进程中的一个核心调控步骤发生在减数分裂前期的粗线期。我们发现，Ndt80在酵母配子发生（孢子形成）的粗线期发挥作用，以激活一组减数分裂（如B型细胞周期蛋白）和配子形成（如SPS1）所需基因的转录。在营养细胞中异位合成Ndt80会诱导这些基因的转录，并且重组Ndt80蛋白会结合到其上游区域的保守序列上。NDT80自身的转录依赖于Ime1，Ime1可激活早期孢子形成基因的表达。Ndt80调控基因CLB1的转录由检查点基因RAD17介导。因此，Ndt80是编程酵母配子发生的转录级联反应的关键组成部分，也可能是减数分裂检查点控制的靶点。
