Gereben B, Leuhuber K, Rausch W D, Gálfi P, Jancsik V, Rudas P
University of Veterinary Science, Dept. of Physiology and Biochemistry, Budapest, Hungary.
Neurobiology (Bp). 1998;6(2):141-50.
Vimentin contributes to the cytoskeleton of different cell-types, among them glial cells. We report here that different forms of this protein, distinguishable by the monoclonal antibodies Vim3B4 and V9, are species-specifically expressed in cultures of glial fibrillary acidic protein (GFAP) positive, primary astrocytes of the chicken and rat. Most cells in the cultures co-expressed GFAP and one of the two vimentin epitopes. The Vim3B4 positive epitope was present in chicken astrocytes, while the V9 positive was not. Inverse situation was found in the astrocytes of rat. In vitro age of the cells did not influence the hierarchy of vimentin epitope expression with respect to species-specificity. Our result shows that the different vimentin expression program of cultured astrocytes of the chicken and rat is preserved under in vitro conditions. The presented data support the concept of the species-specific regulation of vimentin forms in glial cells of the central nervous system.
波形蛋白参与不同细胞类型的细胞骨架构成，其中包括神经胶质细胞。我们在此报告，这种蛋白的不同形式，可通过单克隆抗体Vim3B4和V9区分，在鸡和大鼠的胶质纤维酸性蛋白（GFAP）阳性的原代星形胶质细胞培养物中呈物种特异性表达。培养物中的大多数细胞共表达GFAP和两种波形蛋白表位之一。Vim3B4阳性表位存在于鸡星形胶质细胞中，而V9阳性表位则不存在。在大鼠星形胶质细胞中发现了相反的情况。细胞的体外培养年龄并不影响波形蛋白表位表达在物种特异性方面的层次结构。我们的结果表明，鸡和大鼠培养的星形胶质细胞中不同的波形蛋白表达程序在体外条件下得以保留。所呈现的数据支持中枢神经系统神经胶质细胞中波形蛋白形式存在物种特异性调节的概念。
