Ananthan S, Saravanan P
Department of Microbiology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai.
Indian J Med Res. 1998 Aug;108:58-61.
To determine the individual human rotavirus serotypes prevailing in Chennai, 345 stool specimens obtained from children with acute diarrhoea between March 1996 and November 1997, were screened for the presence of rotavirus by the standardized enzyme linked immunosorbent assay (ELISA). Of the 90 (26%) rotavirus positive specimens, 75 (83.3%) were subgrouped and 65 (72.2%) were serotyped with monoclonal antibody based ELISA. Of the 65 specimens that could be serotyped, 52.3 per cent belonged to serotype 2, 24.6 per cent were serotype 4, 15.4 per cent were serotype 1 and 7.7 per cent were serotype 3. Of the 75 specimens typed for their subgroup specificity, 50.7 per cent were subgroup I, 26.7 per cent were subgroup II, 18.7 per cent were equally specific to both subgroups I and II, 4 per cent belonged to nonsubgroups I and II. Our results indicate a predominance of serotype 2 virus. Unusual strain having both subgroups I and II specificity or neither specificity were also encountered.
为确定钦奈地区流行的人轮状病毒各血清型，我们采用标准化酶联免疫吸附试验（ELISA），对1996年3月至1997年11月期间从患急性腹泻儿童中采集的345份粪便标本进行了轮状病毒筛查。在90份（26%）轮状病毒阳性标本中，75份（83.3%）进行了亚组分型，65份（72.2%）采用基于单克隆抗体的ELISA进行了血清型分型。在65份可进行血清型分型的标本中，52.3%属于血清型2，24.6%为血清型4，15.4%为血清型1，7.7%为血清型3。在75份进行亚组特异性分型的标本中，50.7%为I亚组，26.7%为II亚组，18.7%对I亚组和II亚组具有同等特异性，4%不属于I亚组和II亚组。我们的结果表明血清型2病毒占主导地位。同时还发现了具有I亚组和II亚组特异性或无特异性的异常毒株。
