印度东部感染人类的A组轮状病毒株的基因组多样性。
Genomic diversity of group A rotavirus strains infecting humans in eastern India.
作者信息
Das Soma, Sen A, Uma G, Varghese V, Chaudhuri S, Bhattacharya S K, Krishnan T, Dutta P, Dutta D, Bhattacharya M K, Mitra U, Kobayashi N, Naik T N
机构信息
Division of Virology, National Institute of Cholera and Enteric Diseases, Kolkata, India.
出版信息
J Clin Microbiol. 2002 Jan;40(1):146-9. doi: 10.1128/JCM.40.1.146-149.2002.
Between 1998 and 2000, a total of 266 samples were found positive for group A rotaviruses by RNA electrophoresis. Samples were collected from patients admitted to two leading hospitals in Calcutta. Serotyping could be done only with 22% of the positive samples, leaving 78% untypeable. The G (VP7 genotypes) and P (VP4 genotypes) types were determined for 159 samples by reverse transcription and multiplex PCR. The predominant genotype was G1P[8] (20%), followed by G2P[4] (15%) and G4P[8] (6%). A number of uncommon genotypes, G1P[4] (4%), G2P[8] (2.5%), G2P[6] (0.6%), G4P[4] (2.5%), and G4P[6] (1.25%), were also detected during this study period. Twenty two percent of specimens showed mixed infections, 38 (24%) of the total samples remained untypeable for either VP7 or VP4, while only 4 (2.5%) of the samples were untypeable for both genes. Eleven specimens collected from Manipur were also genotyped and revealed a very high degree of genomic reassortment.
1998年至2000年间，通过RNA电泳共发现266份样本的A组轮状病毒呈阳性。样本取自加尔各答两家主要医院收治的患者。仅对22%的阳性样本进行了血清分型，其余78%无法分型。通过逆转录和多重PCR对159份样本确定了G（VP7基因型）和P（VP4基因型）类型。主要基因型为G1P[8]（20%），其次是G2P[4]（15%）和G4P[8]（6%）。在本研究期间还检测到一些不常见的基因型，G1P[4]（4%）、G2P[8]（2.5%）、G2P[6]（0.6%）、G4P[4]（2.5%）和G4P[6]（1.25%）。22%的标本显示混合感染，38份（24%）样本的VP7或VP4均无法分型，而仅4份（2.5%）样本的两个基因均无法分型。从曼尼普尔采集的11份标本也进行了基因分型，结果显示基因组重配程度非常高。
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