从巴西中部儿童中分离出的轮状病毒血清型和亚组

Serotypes and subgroups of rotavirus isolated from children in central Brazil.

作者信息

Cardoso das D, Soares C M, Azevedo M S, Leite J P, Munford V, Rácz M L

机构信息

Lab. Virologia/IPTSP/UFG-Goiânia-GO, Brazil.

出版信息

J Health Popul Nutr. 2000 Jun;18(1):39-43.

PMID:11014769

Abstract

Group A rotavirus, obtained from children of Goiânia, Brazil, during 1987-1994, were analyzed for subgroup and G serotype by enzyme-linked immunosorbent assay with monoclonal antibodies. The index of serotyping obtained was 61.4% with the following proportions: G1--19.7%, G2--28.0%, G3--9.8%, G4--1.5%, and G5--2.3%. It was observed that G1 occurred from 1987 to 1989 and from 1993 to 1994, and G2 from 1990 to 1993. About 94% of the samples (85/90) could be subgrouped with the following results: 55.5% for SG II, 7.8% SG I, and 31.1% for SG non-I-non-II. Unusual relationship patterns were also detected among serotypes, subgroups, and profiles of electropherotypes in 57.0% of the samples: 20 of them were G2/SG II/"long" profile. The results suggest that variation in temporal and regional characteristics should be considered in the development of rotavirus vaccine.

摘要

1987年至1994年期间从巴西戈亚尼亚儿童中获取的A组轮状病毒，通过使用单克隆抗体的酶联免疫吸附测定法分析其亚组和G血清型。血清分型指数为61.4%，比例如下：G1型——19.7%，G2型——28.0%，G3型——9.8%，G4型——1.5%，G5型——2.3%。观察到G1型出现在1987年至1989年以及1993年至1994年，G2型出现在1990年至1993年。约94%的样本（85/90）可进行亚组划分，结果如下：II型亚组占55.5%，I型亚组占7.8%，非I非II型亚组占31.1%。在57.0%的样本中还检测到血清型、亚组和电泳图谱之间存在异常关系模式：其中20个为G2/II型亚组/“长”型图谱。结果表明，在轮状病毒疫苗的研发中应考虑时间和区域特征的变化。