一种标记石房蛤毒素及其与兔迷走神经、龙虾步足和雀鳝嗅神经无髓纤维结合的新方法。
A new method for labelling saxitoxin and its binding to non-myelinated fibres of the rabbit vagus, lobster walking leg, and garfish olfactory nerves.
作者信息
Ritchie J M, Rogart R B, Strichartz G R
出版信息
J Physiol. 1976 Oct;261(2):477-94. doi: 10.1113/jphysiol.1976.sp011569.
Abstract
- A new method of labelling saxitoxin (STX) is described, based on transfer of tritium from tritiated water to the toxin. 2. The radiochemical purity of the labelled toxin has been directly determined, rather than being based on indirect biochemical means, as in previous experiments with Wilzbach-labelled STX and TTX. 3. The specific activity of the labelled toxin, 66 d..m.f-mole-1, corresponds with one tritium atom per molecule STX, an improvement of about 300-fold over other means of labelling TTX and STX. 4. The binding of this toxin to rabbit, lobster and garfish olfactory nerve fibres has been re-examined. 5. The density of sodium channels calculated on the basis of the binding of the toxin is about four to six-times the values previously reported.
摘要
- 描述了一种标记石房蛤毒素（STX）的新方法，该方法基于氚从氚化水转移到毒素上。2. 已直接测定了标记毒素的放射化学纯度，而不是像之前用威尔茨巴赫法标记STX和TTX的实验那样基于间接生化手段。3. 标记毒素的比活为66 d..m.f-mole-1，相当于每个STX分子有一个氚原子，比其他标记TTX和STX的方法提高了约300倍。4. 重新研究了这种毒素与兔、龙虾和雀鳝嗅神经纤维的结合。5. 根据毒素结合计算出的钠通道密度约为先前报道值的四到六倍。
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