神经膜中钠通道数量的上限？
An upper limit to the number of sodium channels in nerve membrane?
作者信息
Moore J W, Narahashi T, Shaw T I
出版信息
J Physiol. 1967 Jan;188(1):99-105. doi: 10.1113/jphysiol.1967.sp008126.
Abstract
- A small volume of artificial sea water containing 300 nM tetrodotoxin (TTX) was applied successively to seven lobster nerve trunks and the cumulative uptake of toxin investigated by bio-assay.2. Light and electron microscopy indicated that the nerve trunks had a total axonal area of 0.7 x 10(4) cm(2)/g.3. Sodium analysis gave a sodium space for the nerve trunks of 30%.4. The amount of toxin taken up by the cells in 1 g of nerve is less than 1.6 x 10(-11) moles.5. It is argued that there are probably fewer than 13 sodium channels/mu(2) axon in lobster nerve.
摘要
将含有300纳摩尔河豚毒素（TTX）的少量人工海水依次施加到七条龙虾神经干上，并通过生物测定法研究毒素的累积摄取情况。
光学显微镜和电子显微镜检查表明，神经干的轴突总面积为0.7×10⁴平方厘米/克。
钠分析得出神经干的钠空间为30%。
1克神经中的细胞摄取的毒素量少于1.6×10⁻¹¹摩尔。
有人认为，龙虾神经中每平方微米轴突的钠通道可能少于13个。
相似文献
1
An upper limit to the number of sodium channels in nerve membrane?神经膜中钠通道数量的上限？
J Physiol. 1967 Jan;188(1):99-105. doi: 10.1113/jphysiol.1967.sp008126.
2
The binding of tetrodotoxin to nerve membranes.河豚毒素与神经膜的结合。
J Physiol. 1971 Feb;213(1):235-54. doi: 10.1113/jphysiol.1971.sp009379.
3
Pharmacological modifications of the sodium channels of frog nerve.青蛙神经钠通道的药理学修饰
J Gen Physiol. 1968 Feb;51(2):199-219. doi: 10.1085/jgp.51.2.199.
4
Mechanism of nerve membrane depolarization caused by grayanotoxin I.灰藓毒素I引起神经膜去极化的机制。
J Physiol. 1974 Oct;242(2):471-87. doi: 10.1113/jphysiol.1974.sp010718.
5
Crayfish muscle: permeability to sodium induced by calcium depletion.小龙虾肌肉：钙耗竭诱导的对钠的通透性
Science. 1967 Mar 10;155(3767):1263-6. doi: 10.1126/science.155.3767.1263.
6
Ions, drugs and the axon membrane.离子、药物与轴突膜
Ann N Y Acad Sci. 1966 Jul 14;137(2):967-81. doi: 10.1111/j.1749-6632.1966.tb50210.x.
7
Saxitoxin and tetrodotoxin: comparison of nerve blocking mechanism.石房蛤毒素和河豚毒素：神经阻断机制的比较。
Science. 1967 Sep 22;157(3795):1441-2. doi: 10.1126/science.157.3795.1441.
8
Mechanism of action of tetrodotoxin and saxitoxin on excitable membranes.河豚毒素和石房蛤毒素对可兴奋膜的作用机制。
Fed Proc. 1972 May-Jun;31(3):1124-32.
9
The pharmacology of batrachotoxin. II. Effect on electrical properties of the mammalian nerve and skeletal muscle membranes.箭毒蛙毒素的药理学。II. 对哺乳动物神经和骨骼肌膜电特性的影响。
J Pharmacol Exp Ther. 1971 Mar;176(3):511-28.
10
Heterogeneity of excitable membrane: electrophysiological and pharmacological evidence and some consequences.可兴奋膜的异质性：电生理和药理学证据及一些后果。
Ann N Y Acad Sci. 1966 Jul 14;137(2):901-49. doi: 10.1111/j.1749-6632.1966.tb50208.x.
引用本文的文献
1
Ionic channels in nerve membranes, 50 years on.神经膜离子通道：50 年的探索历程。
Prog Biophys Mol Biol. 2022 Mar-May;169-170:12-20. doi: 10.1016/j.pbiomolbio.2021.11.003. Epub 2021 Nov 29.
2
Patch-clamp fluorometry-based channel counting to determine HCN channel conductance.基于膜片钳荧光测定法的通道计数以确定超极化激活环核苷酸门控通道（HCN通道）的电导
J Gen Physiol. 2016 Jul;148(1):65-76. doi: 10.1085/jgp.201511559.
3
A transition state theory approach to the kinetics of conductance changes in excitable membranes.用过渡态理论研究可兴奋细胞膜电导率变化的动力学。
J Membr Biol. 1969 Dec;1(1):248-73. doi: 10.1007/BF01869785.
4
In vitro excitation of purified membrane fragments by cholinergic agonists : III. Comparison of the dose-response curves to decamethonium with the corresponding binding curves of decamethonium to the cholinergic receptor.在体外用胆堿能激动剂刺激纯化的膜片段：III. 脒甲酰胆碱的剂量-反应曲线与脒甲酰胆碱对胆堿能受体的相应结合曲线的比较。
J Membr Biol. 1971 Mar;6(1):58-80. doi: 10.1007/BF01874114.
5
The thickness, composition and structure of some lipid bilayers and natural membranes.一些类脂双层膜和天然膜的厚度、组成和结构。
J Membr Biol. 1971 Sep;5(3):277-96. doi: 10.1007/BF01870555.
6
The Hodgkin-Huxley heritage: from channels to circuits.《霍奇金-赫胥黎遗产：从通道到电路》
J Neurosci. 2012 Oct 10;32(41):14064-73. doi: 10.1523/JNEUROSCI.3403-12.2012.
7
Tetrodotoxin: a brief history.河豚毒素：简史
Proc Jpn Acad Ser B Phys Biol Sci. 2008;84(5):147-54. doi: 10.2183/pjab.84.147.
8
Seventh W.D.M. Paton Memorial Lecture. The man who never was--Walter Ernest Dixon FRS.第七届W.D.M. 佩顿纪念讲座。从未存在过的人——沃尔特·欧内斯特·迪克森，皇家学会会员
Br J Pharmacol. 2001 Aug;133(7):945-50. doi: 10.1038/sj.bjp.0704175.
9
Detection of tetrodotoxin in autopsy material by gas chromatography.通过气相色谱法检测尸检材料中的河豚毒素。
Arch Toxicol. 1980 Apr;44(4):291-7. doi: 10.1007/BF00278036.
10
Sodium currents in the giant axon of the crab Carcinus maenas.海蟹（Carcinus maenas）巨轴突中的钠电流。
J Membr Biol. 1982;66(3):159-69. doi: 10.1007/BF01868491.
本文引用的文献
1
Current-voltage relations in the lobster giant axon membrane under voltage clamp conditions.电压钳制条件下龙虾巨轴突膜的电流-电压关系
J Gen Physiol. 1962 Jul;45(6):1217-38. doi: 10.1085/jgp.45.6.1217.
2
TETRODOTOXIN BLOCKAGE OF SODIUM CONDUCTANCE INCREASE IN LOBSTER GIANT AXONS.河豚毒素对龙虾巨轴突中钠电导增加的阻断作用
J Gen Physiol. 1964 May;47(5):965-74. doi: 10.1085/jgp.47.5.965.
3
TARICHATOXIN--TETRODOTOXIN: A POTENT NEUROTOXIN.蛤蚌毒素——河豚毒素：一种强效神经毒素。
Science. 1964 May 29;144(3622):1100-10. doi: 10.1126/science.144.3622.1100.
4
The positive and negative heat production associated with a nerve impulse.与神经冲动相关的正热产生和负热产生。
Proc R Soc Lond B Biol Sci. 1958 Feb 18;148(931):149-87. doi: 10.1098/rspb.1958.0012.
5
Blockage of sodium conductance increase in lobster giant axon by tarichatoxin (tetrodotoxin).河豚毒素对龙虾大神经轴突钠电导增加的阻断作用。
J Gen Physiol. 1966 May;49(5):977-88. doi: 10.1085/jgp.49.5.977.
6
Voltage clamp experiments on internally perfused giant axons.对内部灌注的巨大轴突进行电压钳实验。
J Physiol. 1965 Oct;180(4):788-820. doi: 10.1113/jphysiol.1965.sp007732.
7
The action of tetrodotoxin on electrogenic components of squid giant axons.河豚毒素对乌贼巨大轴突电生成成分的作用。
J Gen Physiol. 1965 Jul;48(6):975-96.
Zotero 插件