增殖性糖尿病视网膜病变、增殖性玻璃体视网膜病变及黄斑皱襞视网膜前膜中血管内皮生长因子和肿瘤坏死因子α的检测

Detection of vascular endothelial growth factor and tumor necrosis factor alpha in epiretinal membranes of proliferative diabetic retinopathy, proliferative vitreoretinopathy and macular pucker.

作者信息

Armstrong D, Augustin A J, Spengler R, Al-Jada A, Nickola T, Grus F, Koch F

机构信息

Department of Clinical Laboratory Science and Pathology, University of Buffalo, N.Y., USA.

出版信息

Ophthalmologica. 1998;212(6):410-4. doi: 10.1159/000027378.

DOI:10.1159/000027378

PMID:9787233

Abstract

PURPOSE

To evaluate epiretinal membranes in proliferative eye disease for the presence of vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF-alpha).

METHODS

Membranes were surgically removed from 66 patients with proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR) and macular pucker (MP). Cytokine concentrations were determined by ELISA (VEGF) and bioassay (TNF-alpha).

RESULTS

VEGF was detected in all 66 membranes investigated. The highest VEGF values were found in patients with type I diabetes (mean = 5,994 pg/mg protein). In patients with type II diabetes, the values were at a mean of 1,242 pg/mg protein. When coagulation therapy was performed for longer than 3 months prior to surgery, VEGF was significantly (p < 0.05) reduced. Intermediate levels of VEGF were found in PVR membranes (mean = 1,417 pg/mg protein). The lowest activity was found in MP (mean = 216 pg/mg protein). In contrast, TNF-alpha was present in 16 PDR membranes, 9 PVR membranes and 8 MP membranes.

CONCLUSION

The presence of VEGF in all membranes investigated indicates that this cytokine plays an important role in angiogenesis in ischemic retinal disease and in membrane growth in proliferative disorders.

摘要

目的

评估增生性眼病视网膜前膜中血管内皮生长因子（VEGF）和肿瘤坏死因子α（TNF-α）的存在情况。

方法

从66例增生性糖尿病视网膜病变（PDR）、增生性玻璃体视网膜病变（PVR）和黄斑皱襞（MP）患者中手术切除视网膜前膜。通过酶联免疫吸附测定法（ELISA）（检测VEGF）和生物测定法（检测TNF-α）测定细胞因子浓度。

结果

在所研究的所有66个视网膜前膜中均检测到VEGF。I型糖尿病患者的VEGF值最高（平均 = 5,994 pg/mg蛋白）。II型糖尿病患者的值平均为1,242 pg/mg蛋白。术前进行超过3个月的凝血治疗时，VEGF显著降低（p < 0.05）。PVR视网膜前膜中发现VEGF处于中等水平（平均 = 1,417 pg/mg蛋白）。MP中活性最低（平均 = 216 pg/mg蛋白）。相比之下，16个PDR视网膜前膜、9个PVR视网膜前膜和8个MP视网膜前膜中存在TNF-α。