噬菌体T5 DNA中两个异亮氨酸tRNA同工受体基因的定位
Mapping of two isoleucine tRNA isoacceptor genes in bacteriophage T5 DNA.
作者信息
Hunt C, Hwang L T, Weiss S B
出版信息
J Virol. 1976 Oct;20(1):63-9. doi: 10.1128/JVI.20.1.63-69.1976.
Abstract
T5 bacteriophage codes for the synthesis of more than 14 different tRNA species, which map in four separate clusters in the C segment of the T5 chromosome. In this study, two tRNAile isoacceptor species have been identified by reverse-phase chromatography and shown to be transcribed from two different tRNA loci along the T5 chromosome. The map positions of the tRNA isoacceptors were aided by the use of several T5 deletion mutants in which the position and size of the deleted DNA segments had been previously determined by heteroduplex mapping. Hybridization analysis suggests the presence of some sequence homology between the two tRNAile species.
摘要
T5噬菌体编码合成14种以上不同的tRNA种类，这些tRNA种类定位在T5染色体C段的四个独立簇中。在本研究中，通过反相色谱法鉴定出两种异受体tRNAile种类，并表明它们是从T5染色体上的两个不同tRNA基因座转录而来的。利用几个T5缺失突变体辅助确定了异受体tRNA的图谱位置，其中缺失DNA片段的位置和大小先前已通过异源双链体图谱确定。杂交分析表明两种tRNAile种类之间存在一些序列同源性。
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