Fukuda T, Ichimura E, Shinozaki T, Sano T, Kashiwabara K, Oyama T, Nakajima T, Nakamura T
Second Department of Pathology, Gunma University School of Medicine, Maebashi, Japan.
Pathol Int. 1998 Oct;48(10):757-62. doi: 10.1111/j.1440-1827.1998.tb03834.x.
To understand the interaction between hepatocyte growth factor (HGF) and its receptor c-Met on various bone and soft tissue tumors, their expressions were investigated by western blot analysis, immunohistochemistry and enzyme immunoassay. Western blot analysis revealed that c-Met protein was expressed in 21 (38.8%) of 54 tumors, which detailed to seven (25.9%) of 27 bone tumors and 14 (51.8%) of 27 soft tissue tumors. Most malignant fibrous histiocytomas (MFH) and all neurofibromas expressed c-Met protein. The highest expression of c-Met protein was seen in a case of biphasic synovial sarcoma, where its immunoreactivity was localized only on the epithelial component and not on the sarcomatous component. By enzyme immunoassay for HGF, all but one MFH showed HGF production and the mean level of HGF was the highest among the tumors investigated. Neurofibromas and osteosarcomas had the next highest mean levels of HGF production, respectively. Coexpression of HGF and c-Met was observed in 19 (35.2%) of 54 tumors and was frequently observed in neurofibroma, followed by MFH and synovial sarcoma. Although the mode of interaction between HGF and c-Met varies among the various bone and soft tissue tumors including MFH, their signaling system may play an important role in the development and progression of bone and soft tissue tumors.
为了解肝细胞生长因子（HGF）及其受体c-Met在各种骨和软组织肿瘤中的相互作用，通过蛋白质印迹分析、免疫组织化学和酶免疫测定法对它们的表达进行了研究。蛋白质印迹分析显示，54个肿瘤中有21个（38.8%）表达c-Met蛋白，其中27个骨肿瘤中有7个（25.9%），27个软组织肿瘤中有14个（51.8%）。大多数恶性纤维组织细胞瘤（MFH）和所有神经纤维瘤均表达c-Met蛋白。在一例双相滑膜肉瘤中观察到c-Met蛋白的最高表达，其免疫反应性仅定位于上皮成分，而非肉瘤成分。通过对HGF的酶免疫测定，除一个MFH外，所有MFH均显示有HGF产生，且HGF的平均水平在所研究的肿瘤中最高。神经纤维瘤和骨肉瘤的HGF产生平均水平分别次之。在54个肿瘤中有19个（35.2%）观察到HGF和c-Met的共表达，且在神经纤维瘤中经常观察到，其次是MFH和滑膜肉瘤。尽管HGF和c-Met之间的相互作用模式在包括MFH在内的各种骨和软组织肿瘤中有所不同，但其信号系统可能在骨和软组织肿瘤的发生和发展中起重要作用。
