Hamada S, Ooshima T, Masuda N, Mizuno J, Sobue S
Jpn J Microbiol. 1976 Aug;20(4):321-30. doi: 10.1111/j.1348-0421.1976.tb00994.x.
Dextranase AD17 obtained from a culture liquor of a strain of Spicaria violacea was assessed for its ability to inhibit the development of dental caries in conventional Sprague-Dawley rats which had been infected with one of the Streptococcus mutans strains. MT6R (serotype c), OMZ 176R (d), or MT-703R (e). These experiments showed that caries was significantly inhibited when rats were given cariogenic diets No. 2000 and drinking water containing AD17 at a concentration of 10 units/g, as compared to control rats not given dextranase. The inhibitory effects of AD17 were more prominent in smooth surface caries than in total caries. AD17 had a tendency to retard both the establishment of inoculated S. mutans and plaque deposition on tooth surfaces. However, S. mutans could be implanted in the rat oral cavity after repeated inoculation of the bacteria, even in the presence of AD17. These results suggest that the anticaries activity of AD17 is due to not only inhibition of adherence of S. mutans cells on tooth surfaces but also to physiochemical changes of dental plaque formed under the enzymatic action of AD17. Preliminary histophatological examination showed that AD17 had no significant toxicity in rats.
对从紫色链霉菌菌株培养液中获得的葡聚糖酶AD17进行评估，以确定其对感染变形链球菌菌株MT6R（血清型c）、OMZ 176R（血清型d）或MT - 703R（血清型e）之一的常规斯普拉格-道利大鼠龋齿发展的抑制能力。这些实验表明，与未给予葡聚糖酶的对照大鼠相比，当给大鼠喂食致龋性2000号饲料并饮用含浓度为10单位/克AD17的水时，龋齿得到显著抑制。AD17对光滑面龋齿的抑制作用比对总龋齿的抑制作用更显著。AD17倾向于延缓接种的变形链球菌的定植以及牙面菌斑的沉积。然而，即使存在AD17，在反复接种细菌后变形链球菌仍可植入大鼠口腔。这些结果表明，AD17的防龋活性不仅归因于抑制变形链球菌细胞在牙面的黏附，还归因于在AD17酶促作用下形成的牙菌斑的理化变化。初步组织病理学检查表明，AD17对大鼠无明显毒性。
