Lu J X, Gallur A, Flautre B, Anselme K, Descamps M, Thierry B, Hardouin P
Institut de Recherche sur les Maladies du Squelette, Berck-Sur-Mer, France.
J Biomed Mater Res. 1998 Dec 5;42(3):357-67. doi: 10.1002/(sici)1097-4636(19981205)42:3<357::aid-jbm3>3.0.co;2-i.
In order to understand the influence of the implantation site on bone biomaterial evaluation, we implanted cylinders of HA and beta-TCP ceramics in the femoral diaphysis and condyle of rabbits. After 3, 8, 12, and 24 weeks of implantation, histological investigation and histomorphometry were performed on undecalcified samples. Our results show that spontaneous bone healing in the empty cavities is significantly different (p < 0.05) between cortical (SBH > 80%) and cancellous bone sites (SBH < 31%) and that no new bone is formed in marrow tissue. For both porous ceramics, the highest osteogenesis was obtained in the cortical site. Osteogenesis was intermediate in the cancellous site and weak in the medullar site. The material biodegradation was the strongest in the medullar site and higher in the cancellous site than in the cortical site. Both activities were better in the beta-TCP than in the HA (p > 0.05). The marrow tissue presents a foreign-body reaction more reliable, sensitive, and durable than other bone tissues. Therefore, the cancellous bone site is a good site for evaluation of the biofunctionality of biomaterials because of the equilibrium of the osteogenesis and the biodegradation activities, but marrow tissue seems to be better for testing material biocompatibility in vivo.
为了解植入部位对骨生物材料评估的影响，我们将羟基磷灰石（HA）和β-磷酸三钙（β-TCP）陶瓷圆柱体植入兔股骨干和髁部。植入3、8、12和24周后，对未脱钙样本进行组织学研究和组织形态计量学分析。我们的结果表明，皮质骨（自发性骨愈合>80%）和松质骨部位（自发性骨愈合<31%）的空洞内自发性骨愈合存在显著差异（p<0.05），并且骨髓组织中未形成新骨。对于两种多孔陶瓷，皮质骨部位的成骨作用最强。松质骨部位的成骨作用中等，骨髓部位的成骨作用较弱。材料在骨髓部位的生物降解作用最强，在松质骨部位的生物降解作用高于皮质骨部位。β-TCP的这两种活性均优于HA（p>0.05）。骨髓组织呈现出比其他骨组织更可靠、敏感和持久的异物反应。因此，由于成骨作用和生物降解活性的平衡，松质骨部位是评估生物材料生物功能的良好部位，但骨髓组织似乎更适合在体内测试材料的生物相容性。
