Timares L, Takashima A, Johnston S A
Department of Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75235-8573, USA.
Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13147-52. doi: 10.1073/pnas.95.22.13147.
Dendritic cells (DCs) instruct and activate a naive immune system to mount a response toward foreign proteins. Therefore, it has been hypothesized that an ideal vaccine strategy would be to directly introduce genes encoding antigens into DCs. To test this strategy quantitatively, we have compared the immune response elicited by a genetically transfected DC line to that induced by a fibroblast line, or standard genetic immunization. We observe that a single injection of 500-1,000 transfected DCs can produce a response comparable to that of standard genetic immunization, whereas fibroblasts, with up to 50-fold greater transfection efficiency, were less potent. We conclude that transfection of a small number of DCs is sufficient to initiate a wide variety of immune responses. These results indicate that targeting genes to DCs will be important for controlling and augmenting the immunological outcome in genetic immunization.
树突状细胞（DCs）指导并激活幼稚免疫系统对外源蛋白产生应答。因此，有人提出一种理想的疫苗策略是将编码抗原的基因直接导入DCs。为了定量测试该策略，我们比较了基因转染的DC细胞系引发的免疫应答与成纤维细胞系或标准基因免疫诱导的免疫应答。我们观察到，单次注射500 - 1000个转染的DCs可产生与标准基因免疫相当的应答，而成纤维细胞的转染效率高达其50倍，但效力较低。我们得出结论，转染少量DCs足以引发多种免疫应答。这些结果表明，将基因靶向DCs对于控制和增强基因免疫中的免疫结果具有重要意义。
