Meyer-Lindenberg A, Westhoff A, Wohlsein P, Pohlenz J, Nolte I
Klinik für kleine Haustiere, Tierärztlichen Hochschule Hannover.
Berl Munch Tierarztl Wochenschr. 1998 Sep;111(9):344-51.
In the present study, the measurement of the glomerular filtration rate (GFR) in the cat with the aid of an iodine contrast medium clearance with the renalyzer PRX90 is introduced. Investigations on the accuracy of measurement showed that even repeated measurement of plasma samples after two days of storage at room temperature yielded reproducible clearance results. Also, partial dilution of the plasma sample (2 ml with 1 ml Aqua bidest.) to reduce the volume of blood withdrawn still produced reliable results. Further dilution of the plasma volume (1 ml with 2 ml Aqua bidest.) however did not allow for accurate measurements. A total of 59 cats of different age and sex were included in the study. 31 cats had healthy kidneys with urea and creatinine values within the reference range, unchanged urine findings and physiologic urine protein patterns (SDS-PAGE). These cats served as reference group. The GFR reference value ascertained for these animals was 2.1 ml/min/kg BW (mean = 3.45 ml/min/kg with s = +/- 1.0 ml/min/kg). 28 cats had elevated values of urea and creatinine in the blood, as well as partially changed urine findings. For further diagnosis of renal disease, separation of urine proteins was done with the SDS-PAGE in the PhastSystem, which in all cases yielded a pathologic urine protein pattern. In 11 cases the renal disease could be further confirmed by histological investigation. GFR in these patients was clearly lowered compared with healthy cats, with measured values between 0 and 1.8 ml/min/kg. It can be concluded that the renalyzer allows reliable determination of the GFR also in the cat. To what extent measurement of the GFR is also helpful to diagnose nephropathies in the stage of compensation needs to be further investigated. In cats with high grade uremia and a GFR below 1 ml/min however, an exact calculation is not possible, since the accuracy of measurement within this range is inadequate. Thus, in severe disease no correct assessment is possible, and no statement concerning prognosis can be made.
在本研究中，介绍了借助肾分析仪PRX90通过碘造影剂清除率来测量猫的肾小球滤过率（GFR）。对测量准确性的研究表明，即使在室温下储存两天后的血浆样本重复测量也能得到可重复的清除率结果。此外，血浆样本部分稀释（2 ml加1 ml双蒸水）以减少采血量仍能产生可靠结果。然而，血浆体积进一步稀释（1 ml加2 ml双蒸水）则无法进行准确测量。本研究共纳入59只不同年龄和性别的猫。31只猫的肾脏健康，尿素和肌酐值在参考范围内，尿液检查结果正常，尿液蛋白模式生理（SDS-PAGE）。这些猫作为参考组。为这些动物确定的GFR参考值为2.1 ml/min/kg体重（平均值 = 3.45 ml/min/kg，标准差 = +/- 1.0 ml/min/kg）。28只猫血液中尿素和肌酐值升高，尿液检查结果部分改变。为进一步诊断肾脏疾病，在PhastSystem中用SDS-PAGE对尿液蛋白进行分离，在所有情况下均产生病理性尿液蛋白模式。11例病例通过组织学检查进一步证实了肾脏疾病。与健康猫相比，这些患者的GFR明显降低，测量值在0至1.8 ml/min/kg之间。可以得出结论，肾分析仪也能可靠地测定猫的GFR。GFR测量在多大程度上有助于诊断代偿期肾病仍需进一步研究。然而，在GFR低于1 ml/min的重度尿毒症猫中，由于该范围内测量准确性不足，无法进行精确计算。因此，在严重疾病中无法进行正确评估，也无法做出关于预后的陈述。
