视黄酸和环磷酸腺苷对NG108-15细胞中胆碱乙酰转移酶和囊泡乙酰胆碱转运体表达的影响。

Influence of retinoic acid and of cyclic AMP on the expression of choline acetyltransferase and of vesicular acetylcholine transporter in NG108-15 cells.

作者信息

Diebler M F, Tomasi M, Meunier F M, Israël M, Dolezal V

机构信息

Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, Gif-sur-Yvette, France.

出版信息

J Physiol Paris. 1998 Oct-Dec;92(5-6):379-84. doi: 10.1016/S0928-4257(99)80009-6.

DOI:10.1016/S0928-4257(99)80009-6

PMID:9789841

Abstract

Treatment of the cholinergic cell line NG108-15 with retinoic acid or cAMP results in an increase of choline acetyltransferase activity (ChAT) whereas none of these agents influences the amount of the vesicular acetylcholine transporter (VAChT) as judged from vesamicol binding and immunoblot studies. We suggest that immaturity of posttranslational events controlling the expression of VAChT protein is responsible for the apparent absence of coregulation of ChAT and VAChT protein expression.

摘要

用视黄酸或环磷酸腺苷（cAMP）处理胆碱能细胞系NG108 - 15会导致胆碱乙酰转移酶活性（ChAT）增加，而根据vesamicol结合和免疫印迹研究判断，这些试剂均不影响囊泡乙酰胆碱转运体（VAChT）的量。我们认为，控制VAChT蛋白表达的翻译后事件不成熟是导致ChAT和VAChT蛋白表达明显缺乏共调节的原因。