评估经络免疫卡支原体检测法在儿科患者中检测肺炎支原体特异性IgM的效果。

Evaluation of Meridian ImmunoCard Mycoplasma test for the detection of Mycoplasma pneumoniae-specific IgM in paediatric patients.

作者信息

Matas L, Domínguez J, De Ory F, García N, Galí N, Cardona P J, Hernández A, Rodrigo C, Ausina V

机构信息

Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Facultad de Medicina de la Universidad Autónoma de Barcelona, Spain.

出版信息

Scand J Infect Dis. 1998;30(3):289-93. doi: 10.1080/00365549850160954.

DOI:10.1080/00365549850160954

PMID:9790139

Abstract

The Meridian ImmunoCard Mycoplasma kit, a 10-min card-based enzyme-linked immunosorbent assay (ELISA) designed to detect immunoglobulin M (IgM) antibodies to Mycoplasma pneumoniae was evaluated. We compared the ImmunoCard with the Fujirebio Serodia Myco II particle agglutination test, as well as with the complement fixation (CF) test to detect M. pneumoniae antibodies in paediatric patients. The ImmunoCard test and Serodia Myco II test agreed in 93.95%, and ImmunoCard test and CF test agreed in 83.51% of the 182 specimens tested. Nine specimens gave negative particle agglutination titres in the acute phase sample, and 28 specimens gave negative CF titres in the acute phase sample, although in the ImmunoCard test they were positive. These results may indicate that the ImmunoCard assay detects lower IgM levels of antibodies than the Serodia Myco II and CF test. The ImmunoCard appears to be a good screening assay test for M. pneumoniae IgM in children in whom M. pneumoniae IgM is found frequently.

摘要

对子午线免疫卡支原体试剂盒进行了评估，这是一种基于卡片的10分钟酶联免疫吸附测定（ELISA），旨在检测肺炎支原体免疫球蛋白M（IgM）抗体。我们将免疫卡与富士瑞必欧血清学支原体II颗粒凝集试验以及补体结合（CF）试验进行比较，以检测儿科患者的肺炎支原体抗体。在182份检测标本中，免疫卡试验与血清学支原体II试验的符合率为93.95%，免疫卡试验与CF试验的符合率为83.51%。9份标本在急性期样本中颗粒凝集滴度为阴性，28份标本在急性期样本中CF滴度为阴性，尽管在免疫卡试验中它们为阳性。这些结果可能表明，免疫卡测定法检测到的抗体IgM水平低于血清学支原体II试验和CF试验。免疫卡似乎是一种很好的筛查试验，用于检测肺炎支原体IgM抗体常见的儿童患者。