Ohgaki K, Iida A, Kasumi F, Sakamoto G, Akimoto M, Nakamura Y, Emi M
Department of Molecular Biology, Institute of Gerontology, Nippon Medical School, Kawasaki, Japan.
Genes Chromosomes Cancer. 1998 Nov;23(3):244-7.
A detailed analysis of loss of heterozygosity (LOH) in breast cancers was performed with 11 microsatellite markers on the long arm of chromosome 21. Among the 142 tumors examined, 44 (31%) showed LOH at one or more loci. Peak LOH frequency was observed on band 21q21. Deletion mapping identified a new commonly deleted region in a 6-cM interval of 21q21 between loci D21S1432 and D21S1437, and raised the possibility that one or more tumor suppressor genes associated with breast cancer may exist in this region. Comparison of these alterations with clinicopathological parameters revealed an association of LOH on 21q with loss of progesterone receptor (P = 0.0013).
利用位于21号染色体长臂上的11个微卫星标记，对乳腺癌中的杂合性缺失（LOH）进行了详细分析。在所检测的142个肿瘤中，44个（31%）在一个或多个位点显示出LOH。在21q21带观察到LOH频率峰值。缺失图谱确定了在21q21上位于位点D21S1432和D21S1437之间6厘摩区间内一个新的常见缺失区域，并增加了该区域可能存在一个或多个与乳腺癌相关的肿瘤抑制基因的可能性。将这些改变与临床病理参数进行比较，发现21q上的LOH与孕激素受体缺失相关（P = 0.0013）。
