Casqueiro J, Gutiérrez S, Bañuelos O, Fierro F, Velasco J, Martín J F
Area of Microbiology, Faculty of Biology, University of León, Institute of Biotechnology INBIOTEC Parque Científico de León, Spain.
Mol Gen Genet. 1998 Sep;259(5):549-56. doi: 10.1007/s004380050847.
A DNA fragment containing a gene homologous to LYS2 gene of Saccharomyces cerevisiae was cloned from a genomic DNA library of Penicillium chrysogenum AS-P-78. It encodes a protein of 1409 amino acids (Mr 154859) with strong similarity to the S. cerevisiae (49.9% identity) Schizosaccharomyces pombe (51.3% identity) and Candida albicans (48.12% identity) alpha-aminoadipate reductases and a lesser degree of identity to the amino acid-activating domains of the non-ribosomal peptide synthetases, including the alpha-aminoadipate-activating domain of the alpha-aminoadipyl-cysteinyl-valine synthetase of P. chrysogenum (12.4% identical amino acids). The lys2 gene contained one intron in the 5'-region and other in the 3'-region, as shown by comparing the nucleotide sequences of the cDNA and genomic DNA, and was transcribed as a 4.7-kb monocistronic mRNA. The lys2 gene was localized on chromosome III (7.5 Mb) in P. chrysogenum AS-P-78 and on chromosome IV (5.6 Mb) in strain P2, whereas the penicillin gene cluster is known to be located in chromosome I in both strains. The lys2-encoded protein is a member of the aminoacyladenylate-forming enzyme family with a reductase domain in its C-terminal region.
从产黄青霉AS-P-78的基因组DNA文库中克隆到一个与酿酒酵母LYS2基因同源的DNA片段。它编码一个含有1409个氨基酸（Mr 154859）的蛋白质，该蛋白质与酿酒酵母（49.9% 一致性）、粟酒裂殖酵母（51.3% 一致性）和白色念珠菌（48.12% 一致性）的α-氨基己二酸还原酶具有高度相似性，与非核糖体肽合成酶的氨基酸激活结构域的一致性较低，包括产黄青霉α-氨基己二酰-半胱氨酰-缬氨酸合成酶的α-氨基己二酸激活结构域（12.4% 相同氨基酸）。通过比较cDNA和基因组DNA的核苷酸序列发现，lys2基因在5'区域含有一个内含子，在3'区域含有另一个内含子，并且转录为一个4.7 kb的单顺反子mRNA。lys2基因在产黄青霉AS-P-78中定位于III号染色体（7.5 Mb），在P2菌株中定位于IV号染色体（5.6 Mb），而青霉素基因簇在这两个菌株中均已知位于I号染色体上。lys2编码的蛋白质是氨基酰腺苷酸形成酶家族的成员，在其C末端区域具有一个还原酶结构域。
