人类Xq24-q26区域22.5兆碱基的YAC/STS整合物理图谱和遗传图谱，分辨率为56千碱基对，相邻序列标签位点间距56千碱基。

Integrated YAC/STS physical and genetic map of 22.5 Mb of human Xq24-q26 at 56-kb inter-STS resolution.

作者信息

Nagaraja R, MacMillan S, Jones C, Masisi M, Pengue G, Porta G, Miao S, Casamassimi A, D'Urso M, Brownstein B, Schlessinger D

机构信息

Laboratory of Genetics, National Institute on Aging, 5600 Nathan Shock Drive, Baltimore, Maryland, 21224-6825, USA.

出版信息

Genomics. 1998 Sep 15;52(3):247-66. doi: 10.1006/geno.1998.5438.

DOI:10.1006/geno.1998.5438

PMID:9790744

Abstract

A yeast artificial chromosome sequence-tagged site-based (YAC/STS) physical map of 22.5 Mb of the Xq24-q26 cytogenetic band region of the human X chromosome has been assembled. DNA coverage includes 857 large-insert clones formatted with 405 STSs to provide ninefold depth of DNA. At five points, no bridging clones have been recovered from 20 X-chromosome equivalents of human DNA in YACs or bacterial clones, but the placement of 25 ("CA")n polymorphic markers permits the ordering of contigs by comparison with the genetic linkage map and radiation hybrid data. The map localizes the X3000 translocation breakpoint and six genes (ANT2, NDUFA1, LAMP2, OCRL, IGSF1, and HDGF) at better than 100-kb resolution. The relatively gene-poor nature of the region is consistent with relatively low uniform 34-42% GC content in STSs across nearly all of the region.

摘要

已构建出人类X染色体Xq24 - q26细胞遗传学带区22.5 Mb的基于酵母人工染色体序列标签位点（YAC/STS）的物理图谱。DNA覆盖范围包括用405个序列标签位点（STS）构建的857个大插入片段克隆，以提供9倍深度的DNA覆盖。在五个位点上，未从20个人类DNA的X染色体等效物的酵母人工染色体（YAC）或细菌克隆中获得桥接克隆，但25个（“CA”）n多态性标记的定位允许通过与遗传连锁图谱和辐射杂种数据比较来对重叠群进行排序。该图谱将X3000易位断点和六个基因（ANT2、NDUFA1、LAMP2、OCRL、IGSF1和HDGF）定位到优于100 kb的分辨率。该区域相对基因贫乏的性质与几乎整个区域中STS相对较低的34 - 42%的均匀鸟嘌呤 - 胞嘧啶（GC）含量一致。