75千碱基对序列标签位点分辨率下的X染色体图谱，揭示了重组和GC含量的极端情况。

X chromosome map at 75-kb STS resolution, revealing extremes of recombination and GC content.

作者信息

Nagaraja R, MacMillan S, Kere J, Jones C, Griffin S, Schmatz M, Terrell J, Shomaker M, Jermak C, Hott C, Masisi M, Mumm S, Srivastava A, Pilia G, Featherstone T, Mazzarella R, Kesterson S, McCauley B, Railey B, Burough F, Nowotny V, D'Urso M, States D, Brownstein B, Schlessinger D

机构信息

Center for Genetics in Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

出版信息

Genome Res. 1997 Mar;7(3):210-22. doi: 10.1101/gr.7.3.210.

DOI:10.1101/gr.7.3.210

PMID:9074925

Abstract

A YAC/STS map of the X chromosome has reached an inter-STS resolution of 75 kb. The map density is sufficient to provide YACs or other large-insert clones that are cross-validated as sequencing substrates across the chromosome. Marker density also permits estimates of regional gene content and a detailed comparison of genetic and physical map distances. Five regions are detected with relatively high G + C, correlated with gene richness; and a 17-Mb region with very low recombination is revealed between the Xq13.3 [XIST] and Xq21.3 XY homology loci.

摘要

一张X染色体的酵母人工染色体/序列标签位点图谱已达到75 kb的序列标签位点间分辨率。图谱密度足以提供经过交叉验证可作为全染色体测序底物的酵母人工染色体或其他大插入片段克隆。标记密度还允许对区域基因含量进行估计，并对遗传图谱距离和物理图谱距离进行详细比较。检测到五个G + C含量相对较高的区域，这与基因丰富度相关；并且在Xq13.3[XIST]和Xq21.3 XY同源位点之间发现了一个重组率极低的17 Mb区域。