Piirainen L, Hovi T, Roivainen M
Enterovirus Laboratory, National Public Health Institute, Helsinki, Finland.
Microb Pathog. 1998 Sep;25(3):131-7. doi: 10.1006/mpat.1998.0221.
We exposed representatives of different enteroviruses to treatments imitating various in vivo environments that they face during infection. Short-term treatment in trypsin or human intestinal fluid regularly resulted in a cleavage of the capsid protein VP1, and in some cases of other capsid proteins as well. Infectivity of the virus preparations was usually not affected but there were two exceptions. Coxsackievirus A9 retained its infectivity as tested in RD cells but showed reduced infectivity in GMK cells, as reported previously. More strikingly, the titre of echovirus 22 was decreased by 2 logs. Overnight incubation in intestinal fluid affected the infectivity of all tested viruses, including polioviruses and coxsackievirus B4. Echovirus 22 and, to a lesser extent, coxsackievirus A9 were also partially inactivated by faecal suspension. After 2 h exposure to pH 2 at 37 degrees C all tested viruses retained their infectivity, but after 24 h all were inactivated. We conclude that the stability of enteroviruses exposed to various natural environments varies significantly, and that echovirus 22, no more classified in the genus Enterovirus, appears relatively more sensitive in body fluids.
我们将不同肠道病毒的代表暴露于模拟它们在感染过程中所面临的各种体内环境的处理条件下。在胰蛋白酶或人肠液中进行短期处理通常会导致衣壳蛋白VP1发生裂解，在某些情况下其他衣壳蛋白也会裂解。病毒制剂的感染性通常不受影响，但有两个例外。如先前报道，柯萨奇病毒A9在RD细胞中测试时保留了其感染性，但在GMK细胞中显示出感染性降低。更引人注目的是，埃可病毒22的滴度降低了2个对数。在肠液中过夜孵育影响了所有测试病毒的感染性，包括脊髓灰质炎病毒和柯萨奇病毒B4。埃可病毒22以及程度较轻的柯萨奇病毒A9也被粪便悬液部分灭活。在37℃下暴露于pH 2 2小时后，所有测试病毒都保留了其感染性，但24小时后全部被灭活。我们得出结论，暴露于各种自然环境中的肠道病毒的稳定性差异很大，并且不再归类于肠道病毒属的埃可病毒22在体液中似乎相对更敏感。
