甲型肝炎病毒的酸稳定性

Acid stability of hepatitis A virus.

作者信息

Scholz E, Heinricy U, Flehmig B

机构信息

Abteilung für Medizinische Virologie und Epidemiologie der Viruskrankheiten, Hygiene-Institut der Universität Tübingen, F.R.G.

出版信息

J Gen Virol. 1989 Sep;70 ( Pt 9):2481-5. doi: 10.1099/0022-1317-70-9-2481.

DOI:10.1099/0022-1317-70-9-2481

PMID:2550575

Abstract

The acid stability of unpurified and highly purified hepatitis A virus (HAV) was tested and compared with that of poliovirus type 1, coxsackievirus types A9 and B1 and echovirus type 9. Only HAV had a high residual infectivity after 2 h of exposure to pH 1 at room temperature, remaining infectious for up to 5 h. At 38 degrees C, pH 1, HAV remained infectious for 90 min. Highly purified HAV was found to be infectious for 8 h at pH 1 and room temperature. This indicates that the increased stability is not due to protection by cellular material attached to the virus, but is a virus-specific marker. Under the same conditions, at pH 1 and room temperature, unpurified and highly purified HAV antigens were traceable for 5 and 4 h respectively.

摘要

对未纯化和高度纯化的甲型肝炎病毒（HAV）的酸稳定性进行了测试，并与1型脊髓灰质炎病毒、A9型和B1型柯萨奇病毒以及9型艾柯病毒进行了比较。在室温下暴露于pH 1环境2小时后，只有HAV仍具有较高的残余感染力，长达5小时仍具传染性。在38摄氏度、pH 1条件下，HAV可保持90分钟的传染性。研究发现，高度纯化的HAV在pH 1和室温条件下8小时仍具传染性。这表明稳定性的提高并非由于附着在病毒上的细胞物质的保护作用，而是一种病毒特异性标志物。在相同条件下，即pH 1和室温时，未纯化和高度纯化的HAV抗原分别可检测5小时和4小时。