Sordello S, Bertrand N, Plouët J
Laboratoire de Biologie Moléculaire Eucaryote, UPR 9006 Centre National de la Recherche Scientifique, 118, Route de Narbonne, Toulouse cedex, 31062, France.
Biochem Biophys Res Commun. 1998 Oct 9;251(1):287-90. doi: 10.1006/bbrc.1998.9328.
Evidence from pathophysiological studies support the concept that embryonic development, tumor progression, and hormonally-regulated tissue masses such as adult prostate and corpus luteum are angiogenesis-dependent. We examined if the prostatic expression of vascular endothelial growth factor (VEGF), the major regulator of normal and pathological angiogenesis, was regulated by testosterone. Northern blot of VEGF messenger ribonucleic acid (mRNA) extracted from a human immortalized epithelial prostatic cell line (PNT1) showed that dihydrotestosterone (DHT) up-regulated VEGF mRNA at a level comparable to that observed upon exposure to growth factors. Polymerase chain reaction of reverse transcribed mRNA demonstrated that the ratio of the two splice variants encoding the 121 and 165 isoforms of VEGF were not affected by DHT. VEGF biological activity, measured in the conditioned medium by radio receptor assay, was increased by DHT. Injection of testosterone in adult rats induced a transient increase of the ventral lobe weight and the specific activity of prostatic VEGF, leading to a 7-fold increase in the prostate content of VEGF.
病理生理学研究的证据支持这样一种概念，即胚胎发育、肿瘤进展以及诸如成年前列腺和黄体等受激素调节的组织块都依赖于血管生成。我们研究了血管内皮生长因子（VEGF）（正常和病理性血管生成的主要调节因子）在前列腺中的表达是否受睾酮调节。从人永生化前列腺上皮细胞系（PNT1）提取的VEGF信使核糖核酸（mRNA）的Northern印迹分析表明，二氢睾酮（DHT）上调VEGF mRNA的水平与暴露于生长因子时观察到的水平相当。逆转录mRNA的聚合酶链反应表明，编码VEGF 121和165异构体的两种剪接变体的比例不受DHT影响。通过放射受体测定法在条件培养基中测得的VEGF生物活性因DHT而增加。给成年大鼠注射睾酮会导致腹叶重量和前列腺VEGF的比活性短暂增加，使前列腺中VEGF含量增加7倍。
