Guemouri L, Ramsden JJ, Tarjus G, Viot P, Talbot J
Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, Michigan, 48202
J Colloid Interface Sci. 1998 Nov 15;207(2):317-323. doi: 10.1006/jcis.1998.5781.
Modeling the kinetics of protein adsorption at solid surfaces is needed to predict protein separations, design biosensors, and determine the body's initial response to foreign objects. We develop, at the particle level, a kinetic model that accounts geometrically for the surface blockage due to adsorption and postadsorption conformational (or orientational) transitions. Proteins are modeled as disk-shaped particles of diameter final sigmaalpha that adsorb irreversibly at random positions onto a surface at a rate kac (c is the concentration of protein in the bulk solution). Adsorption occurs only where the surface is empty. Following adsorption, a particle attempts to spread (symmetrically) to a larger diameter final sigmabeta at a rate ks. Spreading only occurs if no overlap with any previously placed particle would result. A set of equations is developed for determining the time evolution of the adsorbed protein density. These predictions are compared to new experimental data for fibronectin onto silica-titania obtained using optical waveguide lightmode spectroscopy (OWLS). We also discuss the general application of this model to experimental data. Copyright 1998 Academic Press.
为预测蛋白质分离、设计生物传感器以及确定机体对外来物体的初始反应，需要对蛋白质在固体表面的吸附动力学进行建模。我们在粒子层面建立了一个动力学模型，该模型从几何角度考虑了吸附以及吸附后构象（或取向）转变所导致的表面堵塞。蛋白质被建模为直径为最终西格玛阿尔法的盘状粒子，其以速率kac（c为本体溶液中蛋白质的浓度）不可逆地随机吸附到表面。吸附仅发生在表面为空的地方。吸附后，粒子试图以速率ks（对称地）扩展到更大直径最终西格玛贝塔。只有在不会与任何先前放置的粒子发生重叠的情况下才会发生扩展。我们推导了一组用于确定吸附蛋白质密度随时间变化的方程。将这些预测结果与使用光波导光模式光谱法（OWLS）获得的纤连蛋白在二氧化硅 - 二氧化钛上的新实验数据进行了比较。我们还讨论了该模型在实验数据中的一般应用。版权所有1998年学术出版社。
