组织蛋白酶B：一种用于产生聚集蛋白聚糖“金属蛋白酶”裂解新表位的替代蛋白酶。
Cathepsin B: an alternative protease for the generation of an aggrecan 'metalloproteinase' cleavage neoepitope.
作者信息
Mort J S, Magny M C, Lee E R
机构信息
Joint Diseases Laboratory, Shriners Hospital for Children and Department of Surgery, McGill University, 1529 Cedar Avenue, Montreal, Quebec, Canada H3G 1A6.
出版信息
Biochem J. 1998 Nov 1;335 ( Pt 3)(Pt 3):491-4. doi: 10.1042/bj3350491.
Abstract
Previously, only matrix metalloproteinases were believed capable of cleaving the cartilage proteoglycan, aggrecan, between Asn341 and Phe342, to yield a small G1 fragment terminating in the residues VDIPEN. We show that the combined endo- and exopeptidase activities of the cysteine protease, cathepsin B, also generate this epitope, suggesting that it should no longer be considered as an exclusive marker of metalloproteinase activity.
摘要
以前，人们认为只有基质金属蛋白酶能够在天冬酰胺341和苯丙氨酸342之间切割软骨蛋白聚糖（聚集蛋白聚糖），从而产生一个以VDIPEN残基结尾的小G1片段。我们发现，半胱氨酸蛋白酶组织蛋白酶B的内切酶和外切酶联合活性也能产生这一表位，这表明它不应再被视为金属蛋白酶活性的唯一标志物。
相似文献
1
Cathepsin B: an alternative protease for the generation of an aggrecan 'metalloproteinase' cleavage neoepitope.组织蛋白酶B：一种用于产生聚集蛋白聚糖“金属蛋白酶”裂解新表位的替代蛋白酶。
Biochem J. 1998 Nov 1;335 ( Pt 3)(Pt 3):491-4. doi: 10.1042/bj3350491.
2
Quantification of a matrix metalloproteinase-generated aggrecan G1 fragment using monospecific anti-peptide serum.使用单特异性抗肽血清对基质金属蛋白酶产生的聚集蛋白聚糖G1片段进行定量分析。
Biochem J. 1995 Apr 1;307 ( Pt 1)(Pt 1):245-52. doi: 10.1042/bj3070245.
3
Immunolocalization of the cleavage of the aggrecan core protein at the Asn341-Phe342 bond, as an indicator of the location of the metalloproteinases active in the lysis of the rat growth plate.在大鼠生长板裂解过程中发挥活性的金属蛋白酶作用位点处，对聚集蛋白聚糖核心蛋白在天冬酰胺341-苯丙氨酸342键处的裂解进行免疫定位，以此作为其定位指标。
Anat Rec. 1998 Sep;252(1):117-32. doi: 10.1002/(SICI)1097-0185(199809)252:1<117::AID-AR10>3.0.CO;2-R.
4
Aggrecan degradation in human cartilage. Evidence for both matrix metalloproteinase and aggrecanase activity in normal, osteoarthritic, and rheumatoid joints.人软骨中的聚集蛋白聚糖降解。正常、骨关节炎和类风湿性关节中基质金属蛋白酶和聚集蛋白聚糖酶活性的证据。
J Clin Invest. 1997 Jul 1;100(1):93-106. doi: 10.1172/JCI119526.
5
The interglobular domain of cartilage aggrecan is cleaved by PUMP, gelatinases, and cathepsin B.软骨聚集蛋白聚糖的球间结构域被PUMP、明胶酶和组织蛋白酶B切割。
J Biol Chem. 1992 Sep 25;267(27):19470-4.
6
Development of a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments: detection of fragments in human synovial fluids.用于检测金属蛋白酶衍生的聚集蛋白聚糖片段的切割位点特异性单克隆抗体的研制：人滑液中片段的检测
Biochem J. 1995 Aug 15;310 ( Pt 1)(Pt 1):337-43. doi: 10.1042/bj3100337.
7
Mutations in the interglobular domain of aggrecan alter matrix metalloproteinase and aggrecanase cleavage patterns. Evidence that matrix metalloproteinase cleavage interferes with aggrecanase activity.聚集蛋白聚糖球间结构域的突变会改变基质金属蛋白酶和聚集蛋白聚糖酶的切割模式。有证据表明基质金属蛋白酶切割会干扰聚集蛋白聚糖酶的活性。
J Biol Chem. 2000 Oct 20;275(42):33038-45. doi: 10.1074/jbc.275.42.33038.
8
Cleavage of aggrecan at the Asn341-Phe342 site coincides with the initiation of collagen damage in murine antigen-induced arthritis: a pivotal role for stromelysin 1 in matrix metalloproteinase activity.在小鼠抗原诱导性关节炎中，聚集蛋白聚糖在Asn341 - Phe342位点的裂解与胶原蛋白损伤的起始同时发生：基质溶解素1在基质金属蛋白酶活性中起关键作用。
Arthritis Rheum. 1999 Oct;42(10):2074-84. doi: 10.1002/1529-0131(199910)42:10<2074::AID-ANR7>3.0.CO;2-5.
9
Cartilage proteoglycan aggregate is degraded more extensively by cathepsin L than by cathepsin B.与组织蛋白酶B相比，组织蛋白酶L对软骨蛋白聚糖聚集体的降解作用更广泛。
Biochem J. 1990 Mar 1;266(2):569-73.
10
Analysis of aggrecan in human knee cartilage and synovial fluid indicates that aggrecanase (ADAMTS) activity is responsible for the catabolic turnover and loss of whole aggrecan whereas other protease activity is required for C-terminal processing in vivo.对人膝关节软骨和滑液中聚集蛋白聚糖的分析表明，聚集蛋白聚糖酶（ADAMTS）活性是整个聚集蛋白聚糖分解代谢转换和丢失的原因，而体内C端加工则需要其他蛋白酶活性。
Biochem J. 2001 Sep 15;358(Pt 3):615-26. doi: 10.1042/0264-6021:3580615.
引用本文的文献
1
Natural Compounds: Potential Therapeutics for the Inhibition of Cartilage Matrix Degradation in Osteoarthritis.天然化合物：抑制骨关节炎中软骨基质降解的潜在治疗方法。
Life (Basel). 2022 Dec 30;13(1):102. doi: 10.3390/life13010102.
2
The Impact of Inflammatory Stimuli on Xylosyltransferase-I Regulation in Primary Human Dermal Fibroblasts.炎症刺激对原代人皮肤成纤维细胞中木糖基转移酶-I调节的影响
Biomedicines. 2022 Jun 19;10(6):1451. doi: 10.3390/biomedicines10061451.
3
Spatial and chronological localization of septoclasts in the mouse Meckel's cartilage.鼠 Meckel 软骨中破骨细胞的时空定位。
Histochem Cell Biol. 2022 May;157(5):569-580. doi: 10.1007/s00418-022-02085-1. Epub 2022 Feb 23.
4
Cathepsins in neuronal plasticity.神经元可塑性中的组织蛋白酶
Neural Regen Res. 2021 Jan;16(1):26-35. doi: 10.4103/1673-5374.286948.
5
: Physiological Function and Role in Disease Management.生理功能及其在疾病管理中的作用。
Cells. 2020 Jul 13;9(7):1679. doi: 10.3390/cells9071679.
6
The Role of Cysteine Cathepsins in Cancer Progression and Drug Resistance.半胱氨酸组织蛋白酶在癌症进展和耐药性中的作用。
Int J Mol Sci. 2019 Jul 23;20(14):3602. doi: 10.3390/ijms20143602.
7
Cysteine Cathepsins and their Extracellular Roles: Shaping the Microenvironment.半胱氨酸组织蛋白酶及其细胞外作用：塑造微环境。
Cells. 2019 Mar 20;8(3):264. doi: 10.3390/cells8030264.
8
Modulation of Receptor Protein Tyrosine Phosphatase Sigma Increases Chondroitin Sulfate Proteoglycan Degradation through Cathepsin B Secretion to Enhance Axon Outgrowth.调节受体蛋白酪氨酸磷酸酶σ通过组织蛋白酶 B 分泌增加软骨素硫酸盐蛋白聚糖降解，从而增强轴突生长。
J Neurosci. 2018 Jun 6;38(23):5399-5414. doi: 10.1523/JNEUROSCI.3214-17.2018. Epub 2018 May 14.
9
Origin and development of septoclasts in endochondral ossification of mice.小鼠软骨内成骨过程中破骨细胞的起源与发育
Histochem Cell Biol. 2018 Jun;149(6):645-654. doi: 10.1007/s00418-018-1653-1. Epub 2018 Feb 20.
10
Keratan sulfate, a complex glycosaminoglycan with unique functional capability.硫酸角质素，一种具有独特功能的复杂糖胺聚糖。
Glycobiology. 2018 Apr 1;28(4):182-206. doi: 10.1093/glycob/cwy003.
本文引用的文献
1
Crystallization of rat procathepsin B.大鼠组织蛋白酶B前体的结晶
Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):874-5. doi: 10.1107/S0907444996001801.
2
Immunolocalization of the cleavage of the aggrecan core protein at the Asn341-Phe342 bond, as an indicator of the location of the metalloproteinases active in the lysis of the rat growth plate.在大鼠生长板裂解过程中发挥活性的金属蛋白酶作用位点处，对聚集蛋白聚糖核心蛋白在天冬酰胺341-苯丙氨酸342键处的裂解进行免疫定位，以此作为其定位指标。
Anat Rec. 1998 Sep;252(1):117-32. doi: 10.1002/(SICI)1097-0185(199809)252:1<117::AID-AR10>3.0.CO;2-R.
3
Major increase in endopeptidase activity of human cathepsin B upon removal of occluding loop contacts.去除封闭环接触后，人组织蛋白酶B的内肽酶活性大幅增加。
Biochemistry. 1997 Oct 14;36(41):12608-15. doi: 10.1021/bi971264+.
4
Aggrecan degradation in human intervertebral disc and articular cartilage.人椎间盘和关节软骨中的聚集蛋白聚糖降解
Biochem J. 1997 Aug 15;326 ( Pt 1)(Pt 1):235-41. doi: 10.1042/bj3260235.
5
Cathepsin B.组织蛋白酶B
Int J Biochem Cell Biol. 1997 May;29(5):715-20. doi: 10.1016/s1357-2725(96)00152-5.
6
Aggrecan degradation in human cartilage. Evidence for both matrix metalloproteinase and aggrecanase activity in normal, osteoarthritic, and rheumatoid joints.人软骨中的聚集蛋白聚糖降解。正常、骨关节炎和类风湿性关节中基质金属蛋白酶和聚集蛋白聚糖酶活性的证据。
J Clin Invest. 1997 Jul 1;100(1):93-106. doi: 10.1172/JCI119526.
7
Solution structure of the link module: a hyaluronan-binding domain involved in extracellular matrix stability and cell migration.连接模块的溶液结构：一个参与细胞外基质稳定性和细胞迁移的透明质酸结合结构域。
Cell. 1996 Sep 6;86(5):767-75. doi: 10.1016/s0092-8674(00)80151-8.
8
Potency and selectivity of the cathepsin L propeptide as an inhibitor of cysteine proteases.组织蛋白酶L前肽作为半胱氨酸蛋白酶抑制剂的效力和选择性。
Biochemistry. 1996 Jun 25;35(25):8149-57. doi: 10.1021/bi952736s.
9
Demonstration by electrospray mass spectrometry that the peptidyldipeptidase activity of cathepsin B is capable of rat cathepsin B C-terminal processing.通过电喷雾质谱法证明组织蛋白酶B的肽基二肽酶活性能够对大鼠组织蛋白酶B进行C末端加工。
Biochem J. 1993 Sep 15;294 ( Pt 3)(Pt 3):923-7. doi: 10.1042/bj2940923.
10
Cathepsin B in osteoarthritis: cytochemical and histochemical analysis of human femoral head cartilage.组织蛋白酶B在骨关节炎中的作用：人股骨头软骨的细胞化学和组织化学分析
Ann Rheum Dis. 1995 Apr;54(4):289-97. doi: 10.1136/ard.54.4.289.
Zotero 插件