Cherif H, Reusens B, Ahn M T, Hoet J J, Remacle C
Laboratoire de Biologie Cellulaire, World Health Organization Collaborating Center for the Development of the Biology of the Endocrine Pancreas, Université Catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium.
J Endocrinol. 1998 Nov;159(2):341-8. doi: 10.1677/joe.0.1590341.
An isocaloric low-protein (LP) diet (8% instead of 20% in controls) given to dams during gestation reduces the fractional insulin release of stimulated fetal islets. The LP diet lowers the plasma concentration of taurine in both pregnant rats and their fetuses. This study reports the effect of taurine on the in vitro release of insulin from control and LP fetal islets. Direct stimulation with taurine, methionine or leucine increased the release of insulin from control islets. Nevertheless, no effect on LP islets was observed with either taurine or methionine. The release of insulin from LP islets was reduced with leucine. The in vitro addition of taurine (0. 3 or 3 mM) to the culture medium increased the release of insulin from the control islets in response to arginine or leucine, but it did not restore the reduced responsiveness of LP islets to these amino acids. When 2.5% taurine was added to the drinking water of control or LP dams (groups C+T and LP+T) throughout gestation, the concentration of taurine increased in the serum of dams and fetuses of both groups. The release of insulin from the LP+T fetuses was restored to control levels when stimulated with taurine, methionine, leucine or arginine. In conclusion, taurine stimulated control fetal islets in vitro, but failed to do so in LP islets. However, the addition of taurine to the diet of LP dams restored to normal the release of insulin from LP fetal islets, indicating the importance of taurine during development for a normal fetal beta cell function.
孕期给母鼠喂食等热量低蛋白（LP）饮食（占比8%，而对照组为20%）会降低受刺激的胎儿胰岛的胰岛素释放分数。LP饮食会降低孕鼠及其胎儿血浆中的牛磺酸浓度。本研究报告了牛磺酸对对照组和LP组胎儿胰岛胰岛素体外释放的影响。用牛磺酸、蛋氨酸或亮氨酸直接刺激可增加对照组胰岛的胰岛素释放。然而，牛磺酸和蛋氨酸对LP组胰岛均无影响。亮氨酸会降低LP组胰岛的胰岛素释放。在培养基中体外添加牛磺酸（0.3或3 mM）可增加对照组胰岛对精氨酸或亮氨酸的胰岛素释放，但并未恢复LP组胰岛对这些氨基酸降低的反应性。在整个孕期，当向对照组或LP组母鼠（C+T组和LP+T组）的饮用水中添加2.5%的牛磺酸时，两组母鼠和胎儿血清中的牛磺酸浓度均升高。当用牛磺酸、蛋氨酸、亮氨酸或精氨酸刺激时，LP+T组胎儿的胰岛素释放恢复到了对照组水平。总之，牛磺酸在体外可刺激对照组胎儿胰岛，但对LP组胰岛无效。然而，在LP组母鼠饮食中添加牛磺酸可使LP组胎儿胰岛的胰岛素释放恢复正常，这表明发育过程中牛磺酸对于正常胎儿β细胞功能很重要。
