Bohach G A, Handley J P, Schlievert P M
Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455.
Infect Immun. 1989 Jan;57(1):23-8. doi: 10.1128/iai.57.1.23-28.1989.
Comparisons of recently published primary sequences of staphylococcal and streptococcal pyrogenic toxins prompted an evaluation of biological and immunological properties of the C terminus of staphylococcal enterotoxin C1. The 59 N-terminal amino acids were deleted from the toxin by digestion with trypsin. The resulting fragment (Mr, 20,659) contained the remaining 180 C-terminal residues. This fragment (Trp F1) consisted of two polypeptide chains (Trp F1a and Trp F1b) linked by cysteine residues. Trp F1 was mitogenic, pyrogenic, and enhanced susceptibility of rabbits to lethal endotoxin shock. In addition, this fragment contained at least one antigenic epitope that cross-reacted with enterotoxin B.
近期发表的葡萄球菌和链球菌热原性毒素一级序列的比较，促使人们对葡萄球菌肠毒素C1 C末端的生物学和免疫学特性进行评估。用胰蛋白酶消化毒素，去除了59个N末端氨基酸。所得片段（Mr，20,659）包含其余180个C末端残基。该片段（Trp F1）由两条通过半胱氨酸残基连接的多肽链（Trp F1a和Trp F1b）组成。Trp F1具有促有丝分裂、致热作用，并增强了兔子对内毒素致死性休克的易感性。此外，该片段包含至少一个与肠毒素B交叉反应的抗原表位。
