Lin G, Zhou K Y, Zhao X G, Wang Z T, But P P
Department of Pharmacology, Chinese University of Hong Kong, Shatin, SAR.
Rapid Commun Mass Spectrom. 1998;12(20):1445-56. doi: 10.1002/(SICI)1097-0231(19981030)12:20<1445::AID-RCM356>3.0.CO;2-G.
The present study describes the determination of two different types of hepatotoxic pyrrolizidine alkaloids (PAs) and also distinguishing the hepatotoxic PAs from non-toxic ones by both in-source collision-induced dissociation high performance liquid chromatography mass spectrometry (CID-HPLC/MS) and HPLC/MS/MS (CID in the collision cell), using electrospray ionization. The mass spectra provided molecular ions and characteristic fragment ions, which could be used readily for a rapid identification of different types of PAs. Applications of both in-source CID-HPLC/MS and HPLC/MS/MS analytical methods were successful for the determination of PAs in blood samples obtained from rats dosed with PAs and in the PA-containing plant. The results demonstrated that the developed HPLC/MS methods with two different CID techniques provided a very simple and rapid analysis for an unequivocal diagnosis of PA poisoning and for definitive identification of PAs in plants or herbal medicines.
本研究描述了两种不同类型肝毒性吡咯里西啶生物碱（PAs）的测定方法，并且通过源内碰撞诱导解离高效液相色谱质谱联用（CID-HPLC/MS）和HPLC/MS/MS（在碰撞池中进行CID），采用电喷雾电离，将肝毒性PAs与无毒PAs区分开来。质谱提供了分子离子和特征碎片离子，可轻松用于快速鉴定不同类型的PAs。源内CID-HPLC/MS和HPLC/MS/MS分析方法在测定经PAs给药大鼠的血液样本以及含PA的植物中的PAs方面均取得成功。结果表明，所开发的采用两种不同CID技术的HPLC/MS方法为明确诊断PA中毒以及最终鉴定植物或草药中的PAs提供了非常简单快速的分析方法。
