一种用于分离胞外多糖缺陷型突变体的新型筛选方法。
A novel screening method for isolating exopolysaccharide-deficient mutants.
作者信息
Liu M, González J E, Willis L B, Walker G C
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
出版信息
Appl Environ Microbiol. 1998 Nov;64(11):4600-2. doi: 10.1128/AEM.64.11.4600-4602.1998.
Abstract
A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria.
摘要
本文描述了一种基于亲脂性染料苏丹黑B对苜蓿根瘤菌（中华根瘤菌）野生型和胞外多糖缺陷型突变体进行差异染色的筛选方法。利用该方法分离出了在琥珀聚糖或EPS II（半乳葡聚糖）产生方面存在缺陷的突变体，该方法可能也有助于分离其他细菌的胞外多糖缺陷衍生物。
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