对从囊性纤维化患者中分离出的洋葱伯克霍尔德菌黏液样菌株所产生的酸性胞外多糖的结构研究。
Structural studies of the acidic exopolysaccharide produced by a mucoid strain of Burkholderia cepacia, isolated from cystic fibrosis.
作者信息
Cérantola S, Marty N, Montrozier H
机构信息
Département des Glycoconjugués et Biomembranes, Laboratoire de Pharmacologie et Toxicologie Fondamentales du CNRS, Toulouse, France.
出版信息
Carbohydr Res. 1996 May 14;285:59-67. doi: 10.1016/s0008-6215(96)90170-6.
The acidic exopolysaccharide produced by a mucoid strain of Burkholderia cepacia isolated from a cystic fibrosis patient, was purified by cetyltrimethylammonium bromide precipitation and/or anion-exchange chromatography. Based on the sugar composition and permethylation analyses, supported by GLC-MS and NMR spectroscopy analyses, the repeating-unit of the polysaccharide was established as -->3)-beta-D-Glcp-(1-->3)-[4,6-O-(1-carboxyethylidene)]-alpha-D-Gal p-(1-->.
从一名囊性纤维化患者分离出的洋葱伯克霍尔德菌黏液样菌株产生的酸性胞外多糖，通过十六烷基三甲基溴化铵沉淀和/或阴离子交换色谱法进行纯化。基于糖组成和全甲基化分析，并得到气相色谱-质谱联用（GLC-MS）和核磁共振光谱（NMR）分析的支持，该多糖的重复单元确定为-->3)-β-D-葡萄糖吡喃糖基-(1-->3)-[4,6-O-(1-羧基亚乙基)]-α-D-半乳糖吡喃糖基-(1-->。
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