猪霍乱沙门氏菌LT2原生质体和透性化细胞对细菌素AS-48的反应
Response of Salmonella choleraesuis LT2 spheroplasts and permeabilized cells to the bacteriocin AS-48.
作者信息
Abriouel H, Valdivia E, Gálvez A, Maqueda M
机构信息
Dpto. Microbiología, Facultad de Ciencias, Universidad de Granada, Fuentenueva s/n, E-18071-Granada, Spain.
出版信息
Appl Environ Microbiol. 1998 Nov;64(11):4623-6. doi: 10.1128/AEM.64.11.4623-4626.1998.
Abstract
The bacteriocin AS-48 was not active against intact cells of Salmonella choleraesuis LT2 at neutral pH, but it was very effective on spheroplasts, suggesting that the outer membrane (OM) acts as a protective barrier. Cells sublethally injured by heat or treated with OM-permeabilizing agents (i.e., EDTA and Tris) became sensitive to AS-48. The combination of two or more treatments decreased the amount of AS-48 required for cell killing. The activity of AS-48 against heat-injured cells did not change significantly in the pH range of 4.0 to 8.0. AS-48 showed bactericidal activity against intact cells of Salmonella at pH 4.0. The potency of AS-48 increased greatly when the bacteriocin was dissolved at pH 9.0.
摘要
细菌素AS-48在中性pH值下对猪霍乱沙门氏菌LT2的完整细胞无活性，但对原生质体非常有效，这表明外膜（OM）起到了保护屏障的作用。受热亚致死损伤或用OM通透剂（即EDTA和Tris）处理的细胞对AS-48变得敏感。两种或更多种处理的组合减少了杀死细胞所需的AS-48量。在4.0至8.0的pH范围内，AS-48对热损伤细胞的活性没有显著变化。AS-48在pH 4.0时对沙门氏菌的完整细胞表现出杀菌活性。当细菌素在pH 9.0溶解时，AS-48的效力大大增加。
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