Irvin R T, MacAlister T J, Costerton J W
J Bacteriol. 1981 Mar;145(3):1397-403. doi: 10.1128/jb.145.3.1397-1403.1981.
The effect of tris(hydroxymethyl)aminomethane (Tris) buffer on outer membrane permeability was examined in a smooth strain (D280) and in a heptose-deficient lipopolysaccharide strain (F515) of Escherichia coli O8. Tris buffer (pH 8.00) was found to increase outer membrane permeability on the basis of an increased Vo of whole-cell alkaline phosphatase activity and on the basis of sensitivity to lysozyme and altered localization pattern of alkaline phosphatase. The Tris buffer-mediated increase in outer membrane permeability was found to be dependent upon the extent of exposure to and concentration of the Tris buffer. The Tris buffer effects were demonstrated not to be due to allosteric activation of cell-associated alkaline phosphatase and were specific for Tris buffer. Exposure of cells to Tris resulted in the release of a limited amount of cell envelope component. Investigators utilizing Tris buffer are cautioned that Tris is not physiologically inert and that it may interact with the system under investigation.
在大肠杆菌O8的光滑菌株（D280）和七糖缺陷型脂多糖菌株（F515）中检测了三（羟甲基）氨基甲烷（Tris）缓冲液对外膜通透性的影响。基于全细胞碱性磷酸酶活性的V0增加、对溶菌酶的敏感性以及碱性磷酸酶定位模式的改变，发现Tris缓冲液（pH 8.00）会增加外膜通透性。发现Tris缓冲液介导的外膜通透性增加取决于暴露于Tris缓冲液的程度和浓度。已证明Tris缓冲液的作用并非由于细胞相关碱性磷酸酶的变构激活，且对Tris缓冲液具有特异性。细胞暴露于Tris会导致有限量的细胞包膜成分释放。使用Tris缓冲液的研究人员需注意，Tris并非生理惰性物质，它可能会与所研究的系统相互作用。
