Sabattini E, Bisgaard K, Ascani S, Poggi S, Piccioli M, Ceccarelli C, Pieri F, Fraternali-Orcioni G, Pileri S A
Haemolymphopathology, Unit, University of Bologna, Italy.
J Clin Pathol. 1998 Jul;51(7):506-11. doi: 10.1136/jcp.51.7.506.
To assess a newly developed immunohistochemical detection system, the EnVision++.
A large series of differently processed normal and pathological samples and 53 relevant monoclonal antibodies were chosen. A chessboard titration assay was used to compare the results provided by the EnVision++ system with those of the APAAP, CSA, LSAB, SABC, and ChemMate methods, when applied either manually or in a TechMate 500 immunostainer.
With the vast majority of the antibodies, EnVision++ allowed two- to fivefold higher dilutions than the APAAP, LSAB, SABC, and ChemMate techniques, the staining intensity and percentage of expected positive cells being the same. With some critical antibodies (such as the anti-CD5), it turned out to be superior in that it achieved consistently reproducible results with differently fixed or overfixed samples. Only the CSA method, which includes tyramide based enhancement, allowed the same dilutions as the EnVision++ system, and in one instance (with the anti-cyclin D1 antibody) represented the gold standard.
The EnVision++ is an easy to use system, which avoids the possibility of disturbing endogenous biotin and lowers the cost per test by increasing the dilutions of the primary antibodies. Being a two step procedure, it reduces both the assay time and the workload.
评估一种新开发的免疫组织化学检测系统——EnVision++。
选取了大量经过不同处理的正常和病理样本以及53种相关单克隆抗体。采用棋盘滴定法，将EnVision++系统与APAAP、CSA、LSAB、SABC和ChemMate方法手动或在TechMate 500免疫染色仪上应用时所提供的结果进行比较。
对于绝大多数抗体，EnVision++允许的稀释倍数比APAAP、LSAB、SABC和ChemMate技术高两到五倍，染色强度和预期阳性细胞百分比相同。对于一些关键抗体（如抗CD5），结果表明它具有优势，因为它对不同固定或过度固定的样本都能始终如一地获得可重复的结果。只有包含酪胺增强的CSA方法允许与EnVision++系统相同的稀释倍数，并且在一个实例中（使用抗细胞周期蛋白D1抗体时）代表了金标准。
EnVision++是一种易于使用的系统，它避免了干扰内源性生物素的可能性，并通过增加一抗的稀释倍数降低了每次检测的成本。作为一种两步法程序，它减少了检测时间和工作量。
