Chilosi M, Lestani M, Pedron S, Montagna L, Benedetti A, Pizzolo G, Menestrina F
Department of Pathology, University of Verona, Italy.
Biotech Histochem. 1994 Jul;69(4):235-9. doi: 10.3109/10520299409106292.
A simple and rapid one-step method for demonstrating immunohistochemical markers (leukocyte common antigen, cytokeratin, etc.) is described, which can help define the nature of poorly differentiated neoplasms for diagnosis using frozen section. Microwave irradiation was used to speed immunohistochemical analysis using "Enhanced Polymer One-step Staining" (EPOS) reagents on cryostat sections from a variety of pathologic samples. Reproducible results were obtained using EPOS reagents for leukocyte common antigen and cytokeratin. The overall procedure takes less than 10 min and can be completed during surgery.
本文描述了一种简单快速的一步法来显示免疫组织化学标志物（白细胞共同抗原、细胞角蛋白等），该方法有助于利用冰冻切片诊断低分化肿瘤的性质。在来自各种病理样本的恒冷箱切片上，使用微波辐射结合“增强聚合物一步法染色”（EPOS）试剂来加速免疫组织化学分析。使用EPOS试剂检测白细胞共同抗原和细胞角蛋白可获得可重复的结果。整个过程耗时不到10分钟，且可在手术过程中完成。
