金鱼视网膜切片中谷氨酸释放的成像。

Imaging of glutamate release from the goldfish retinal slice.

作者信息

Ayoub G S, Dorst K

机构信息

Department of Biology, Westmont College, Santa Barbara, CA 93108, USA.

出版信息

Vision Res. 1998 Oct;38(19):2909-12. doi: 10.1016/s0042-6989(98)00103-5.

DOI:10.1016/s0042-6989(98)00103-5

PMID:9797987

Abstract

A fluorometric procedure to image release of the neurotransmitter glutamate from living retinal slices is described. Patterns of endogenous glutamate efflux were imaged with a cooled CCD camera in goldfish retinal slices as NADH fluorescence produced by a cycling glutamate dehydrogenase (GDH). Basal and potassium evoked glutamate effluxes were strongly localized to the outer and inner plexiform layers, supporting the model that photoreceptors and bipolar cells release glutamate as their prime fast neurotransmitter.

摘要

本文描述了一种用于对活体视网膜切片中神经递质谷氨酸释放进行成像的荧光检测方法。通过循环谷氨酸脱氢酶（GDH）产生的NADH荧光，利用冷却电荷耦合器件（CCD）相机对金鱼视网膜切片中内源性谷氨酸外流模式进行成像。基础和钾诱发的谷氨酸外流强烈定位于外网状层和内网状层，支持了光感受器和双极细胞将谷氨酸作为其主要快速神经递质释放的模型。