Jimbo M, Okubo K, Toma Y, Shimizu Y, Saito H, Yanaihara T
Department of Obstetrics and Gynecology, Showa University, School of Medicine, Tokyo, Japan.
J Obstet Gynaecol Res. 1998 Aug;24(4):291-7. doi: 10.1111/j.1447-0756.1998.tb00092.x.
Aromatization in the the fetal brain is thought to be involved both in sex differentiation during early development and in adult sexual behavior. Although recently the relationship between aromatase and catecholamine has been discussed, the effect of stress on aromatase in the fetal brain has not been clarified. Therefore, in the present study, localization of aromatase and the inhibitory effects of catecholamines and maternal stress on aromatase activity in the fetal rat brain were examined.
Localization of aromatase cytochrome P-450 using a specific polyclonal antiserum against human placenta aromatase was examined, and the inhibitory effects of dopamine and norepinephrine on aromatase activity in vitro were studied. Further, the influences of intrauterine stress on aromatase activity in the prenatal rat brain were evaluated in vivo.
Aromatase-immunoreactive neurons are located principally in the medial amygdaloid nucleus. Aromatase activity in the fetal rat brain was competitively inhibited by dopamine and norepinephrine, with Ki values of 120 microM and 100 microM, respectively. Aromatase activity in the fetal brain was significantly lower in stressed rats given 1.5% salt water (89.2 +/- 17.5 fmol/mg/hr; n = 4) (p < 0.05) than in the control group (123.1 +/- 10.0 fmol/mg/hr; n = 4).
Aromatase activity in the prenatal rat brain is influenced by catecholamine metabolism during intrauterine stress.
胎儿脑内的芳香化作用被认为既参与早期发育过程中的性别分化，也参与成年后的性行为。尽管最近已经讨论了芳香化酶与儿茶酚胺之间的关系，但应激对胎儿脑内芳香化酶的影响尚未阐明。因此，在本研究中，检测了芳香化酶的定位以及儿茶酚胺和母体应激对胎鼠脑内芳香化酶活性的抑制作用。
使用针对人胎盘芳香化酶的特异性多克隆抗血清检测芳香化酶细胞色素P-450的定位，并研究多巴胺和去甲肾上腺素在体外对芳香化酶活性的抑制作用。此外，在体内评估了宫内应激对产前大鼠脑内芳香化酶活性的影响。
芳香化酶免疫反应性神经元主要位于杏仁内侧核。胎鼠脑内的芳香化酶活性受到多巴胺和去甲肾上腺素的竞争性抑制，其Ki值分别为120微摩尔和100微摩尔。给予1.5%盐水的应激大鼠胎脑内的芳香化酶活性（89.2±17.5飞摩尔/毫克/小时；n = 4）（p < 0.05）显著低于对照组（123.1±10.0飞摩尔/毫克/小时；n = 4）。
产前大鼠脑内的芳香化酶活性受宫内应激期间儿茶酚胺代谢的影响。
