Nakhla A M, Rosner W
Department of Medicine, St. Luke's/Roosevelt Hospital Center, New York, New York 10019, USA.
Endocrinology. 1996 Oct;137(10):4126-9. doi: 10.1210/endo.137.10.8828467.
Sex hormone-binding globulin (SHBG) not only regulates the free concentration of certain steroid sex hormones in plasma, but is involved in a nongenomic mechanism of steroid hormone action. It binds to a receptor on prostatic cell membranes and is activated by an appropriate steroid to initiate the generation of intracellular cAMP. Using the human prostate cancer cell line ALVA-41, we show that in serum-free medium, both dihydrotestosterone and estradiol increase growth in the presence, but not the absence, of SHBG. The increase in growth also follows the addition of cAMP to the cells and is enhanced by inhibiting protein dephosphorylation with the protein phosphatase inhibitor, okadaic acid. We conclude that cAMP causes increased growth in this prostate cancer cell line, and that both SHBG-dihydrotestosterone and SHBG-estradiol can regulate intracellular cAMP, and hence growth, in these cells.
性激素结合球蛋白（SHBG）不仅调节血浆中某些类固醇性激素的游离浓度，还参与类固醇激素作用的非基因组机制。它与前列腺细胞膜上的受体结合，并被适当的类固醇激活以启动细胞内cAMP的生成。使用人前列腺癌细胞系ALVA - 41，我们发现，在无血清培养基中，二氢睾酮和雌二醇在有SHBG存在而非不存在时会促进生长。向细胞中添加cAMP后也会出现生长增加，并且通过用蛋白磷酸酶抑制剂冈田酸抑制蛋白去磷酸化而增强。我们得出结论，cAMP导致该前列腺癌细胞系生长增加，并且SHBG - 二氢睾酮和SHBG - 雌二醇均可调节这些细胞内的cAMP，进而调节生长。
