Aguadé M
Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, 08071 Barcelona, Spain.
Genetics. 1998 Nov;150(3):1079-89. doi: 10.1093/genetics/150.3.1079.
The Acp26Aa and Acp26Ab genes that code for male accessory gland proteins are tandemly arranged in the species of the Drosophila melanogaster complex. An approximately 1.6-kb region encompassing both genes has been sequenced in 10, 24, and 18 lines from Spain, Ivory Coast, and Malawi, respectively; the previously studied 10 lines from North Carolina have also been included in the analyses. A total of 110 nucleotide and 4 length polymorphisms were detected. Silent variation for the whole Acp26A region was slightly higher in African than in non-African populations, while for both genes nonsynonymous variation was similar in all populations studied. Based on Fst estimates no major genetic differentiation was detected between East and West Africa, while in general non-African populations were strongly differentiated from both African populations. Comparison of polymorphism and divergence at synonymous and nonsynonymous sites revealed that directional selection acting on amino acid replacement changes has driven the evolution of the Acp26Aa protein in the last 2.5 myr.
编码雄性附腺蛋白的Acp26Aa和Acp26Ab基因在黑腹果蝇复合体的物种中串联排列。分别对来自西班牙、科特迪瓦和马拉维的10个、24个和18个品系中包含这两个基因的约1.6 kb区域进行了测序；分析中还纳入了之前研究过的来自北卡罗来纳州的10个品系。共检测到110个核苷酸多态性和4个长度多态性。整个Acp26A区域的沉默变异在非洲种群中略高于非非洲种群，而在所有研究种群中，两个基因的非同义变异相似。基于Fst估计，未检测到东非和西非之间存在主要的遗传分化，而总体而言，非非洲种群与两个非洲种群都有强烈分化。同义位点和非同义位点的多态性与分化比较表明，在过去250万年中，作用于氨基酸替代变化的定向选择推动了Acp26Aa蛋白的进化。
